RECHERCHE D’UN MILIEU OPTIMAL POUR LA PRODUCTION DE LA -GLUCOSIDASE CHEZ Aspergillus fumigatus (Fresenius) MUTANT albino
Mots-clés :
Cellulase, glucosidase, submerged culture, chemical treatments, optimal medium, lignocellulosic materials, Aspergillus fumigatus albino mutant strainRésumé
Aspergillus fumigatus (Fresenius) mutant albino, isolé dans notre laboratoire et qui a déjà fait l’objet d’une étude sur la production des enzymes cellulolytiques et xylanolytiques, s’est distingué par son hyperproduction en b-glucosidase (enzyme majeure du système cellulolytique). La production de cette dernière, sur différents milieux, a montré qu’il est possible d’améliorer de 8 à 10 fois les rendements en jouant sur la composition en différents éléments nutritifs. Les caractéristiques mises en évidence peuvent ouvrir une application potentielle dans le processus d’hydrolyse de la biomasse lignocellulosique.
La fermentation est réalisée en milieu liquide sous agitation rotative à 150 tours/min, à +45°C sur des résidus lignocellulosiques variés : A. fumigatus (Fresenius) mutant albino présente d’excellentes activités enzymatiques en présence de 4% d’alfa, avec comme source d’azote optimale, le sulfate d’ammonium à 0.8%, sans adjonction d’éléments minéraux autre que le Mg + (MgC12) à 0.1%. La production en milieu optimisé est de 8972 U/ml, correspondant à une amélioration du rendement de 10 fois par rapport au milieu de base (820 U/ml).
Références
- Odier E. et Rouau X, "Les cellulases et les enzymes de dépolymérisation de la lignine", In Hydrolases et Depolymérases-Enzymes d’intérêt industriel, Collection Biochimie appliquée; pp.1999-237, Ed. Gauthier Villars.
- Woodward J. et Wiseman A., "Fungal and other -D-glucosidases- their properties and applications", Enzyme Microb. Technol., vol. 4 (1982).
- Yamanobe T. and Mitsuishi Y., "Purification and properties of a -glucosidase from fungal strain Y-94", Agric. Biol. Chem., vol.53 (12) (1989), pp.3359-3360.
- Anand L. and Vithayathil P.-J., "Purification and properties of -glucosidase from a thermophilic fungus Humicola lanuginosa (Griffon and Maublanc) Bunce", Journal of Fermentation and Bioengineering, vol.67, N°6 (1989), pp.380-386.
- Riou C., Salmon J-M., Vallier M.-J., Gïnata Z. and Barre P., "Purification, characterization, and substrate specificity of a novel highly glucose-tolerant -glucosidase from Aspergillus oryzae", Applied and Environmental Microbiology, vol.64 (1998), pp.3607-3614.
- Kimura I., Yoshioka N. and Tajima S., "Purification and characterization of a -glucosidase with -xylosidase from Aspergillus sojae", J. of Bioscience and Bioengineering, vol. 87, N°4 (1999), pp.538-541.
- Woodward J. and Wiseman A., "Fungal and other -D-glucosidases- Their properties and applications", Enzyme Microb. Technol., V4 (1982), pp.73-79.
- Kitpreechavanich V., Hayashi M. and Nagai S., "Purification and characterization of extracellular -xylosidase and -glucosidase from Aspergillus fumigatus", Agric. Biol. Chem., 50 (1986), pp.1703-1711.
- Yeoh H.H., Tan T.K. and Koh S.K, ".Kinetic properties of -glucosidase from Aspergillus ornatus", Appl. Microbiol. Biotechnol., 25 (1986), pp.25-28.
- Gaikward J.S. and Maheshwari R., "Localisation and release of -glucosidase in the thermophilic and cellulolytic fungus, Sporotrichum thermophile", Experimental Mycology, 18 (1994), pp.300-310.
- Gueguen Y., Chemardin P., Anand A. and Galzy. P., "Comparative study of extracellular and intracellular -glucosidase of a new strain of Zygosaccharomyces bailii, isolated from fermenting agave juice", J. of Applied Bacteriology, 78 (1995), pp.270-280.
- Günata Z. et Vallier M.J.V., "Production of highly glucose-tolerant extracellular -glucosidase by three Aspergillus strains", Biotechnology letters, Vol. 21 (1999), pp.219-223.
- Rudick M.J. and Elbein A.D., "Glycorprotein enzymes secreted by Aspergillus fumigatus: Purification and properties of -glucosidase", J. Biol. Chem., 248 (1973), pp.6505-6513.
- Chesson A., "Maceration of linen flax under anaerobic conditions", J. of Applied Bacteriology, 45(2) (1978), pp.219-230.
- Wood T.M. et Bhat T.M., "Methods for measuring cellulase activities", In Methods in Enzymology, vol.160, pp.87-112, Ed. W.A. Wood and S.T. Kellogs, Academic Press Inc., USA (1988).
- Lowry O.H., Rosebrough. N.J., Farr A.L. and Randall R.J., "Protein measurement with the phenol reagent", J. Biol. Chem., 193 (1951), pp.265-275.
- Nefsaoui et Vanbelle M., "Utilisation des grignons d’olive en alimentation animale dans le bassin méditerranéen", Université Catholique de Louvain, Faculté des Sciences Agronomiques, Belgique, Publication N°38 (1984).
- Saradouni N., Penninckx J.M. and Salhi O., "High level production of thermostable -xylanse, CMC-ase and -glucosidase by an Aspergillus fumigatus (Fresenius) albino mutant strain, Sciences & Technologie, Univ. Constantine, Algérie, N° 12 (1999), pp. 65-70.
- Stewart J.C. and Parry J.B., "Factors influencing the production of cellulase by Aspergillus fumigatus (Fresenius), J. of General Microbiology, 125 (1981), pp.33-39.
- Szczodrak J., "Production of cellulases and xylanases by Trichoderma reesei F-522 on pretreated wheat straw", Acta. Biotechnol., 8 (1988), pp.509-515.
- Shaker H.M., Farid M.A. and El-Diwany A., "Optimization of the composition medium for cellulase and protein biosynthesis by thermophilic Aspergillus fumigatus NRC 272", Enzyme Microb. Technol., vol.6 (1984), pp.212-216.
- Okada G., "Purification and properties of a cellulase from Aspergillus niger", Agric. Biol. Chem.", 49 (1985), pp. 1257-1265.
- Shin C.S., Lee J.P., Lee J.S. and Park S.C., "Enzyme production of Trichoderma reesei Rut C-30 on various lignocellulosic substrates", Applied Biochemistry and Biotechnology, vol.84-86 (2000), pp. 237-245.
- DeVries R.P. and Visser J., Aspergillus enzymes involved in degradation of plant cell wall polysaccharides", Microbiology and Molecular Biology Reviews, vol. 65, N°2 (2001), pp. 497-522.
- Pushalkar S., Rao K.K. and Menon K., "Production of -glucosidase by Aspergillus terreus, Curr. Microbiol., 30 (1995), pp.255-258.
- Faure D., Henrissat B., Ptacek D., Bekri M.A. and Vanderleyden J., The cel Agene, encoding a glycosyl hydrolase family 3 -glucosidase in Azospirillum irakense, is required for optimal growth on cellobiosides", Applied and Environmental Microbiology, vol.67, N°5 (2001), pp.2380-2383.
- Ghosh M., Das A., Mishra A.K. and Nanda G., "Aspergillus sydowii MG 49 is a strong producer of thermostable xylanolytic enzymes", Enzyme Microb. Technol., V15 (1993), pp.703-709.
- Sun R.C., Tomkinson J., MA.P.L et Liang S.F., "Comparative study of hemicelluloses from rice straw by alkali and hydrogen peroxide treatments", Carbohydrates Polymers, 42 (2000 a), pp.111-122.
- Sun R.C., Tomkinson J., Geng Z.C. and Wang N.J., "Comparative studies of hemicelluloses solubilized during the treatments of maïze stems with peracetic acid and hydrogen peroxide", Holzforsching, 54(5) (2000 b), pp.492-496.
- Sun R.C., Tomkinson J., Mao F.C. and Sun F., "Physico-chemical characterization of lignins from rice straw by hydrogen peroxide treatment", J. of Applied Polymer Science, 79 (2001) pp.719-732.
- Esterbauer N., Steiner W., Labuda I., Hermann A. and Hayn. M., "Production of Trichoderma cellulases in laboratory and pilot scale", Bioresource Technology, 36 (1991), pp.51-65.
- Deacon J.W., "Introduction to modern mycology", vol.7, 2nd Edition, Blackwell Scientific Publications, Oxford, London, Edinburgh (1984).
