REGIME HYPERLIPIDIQUE ET PROCESSUS ATHEROSCLEREUX CHEZ RATTUS NORVEGICUS
Mots-clés :
Athérosclérose, oxydation, cellules musculaires lisses aortiquesRésumé
Notre travail a consisté en l'administration d'un jaune d'œuf cuit durant une année chez Rattus norvegicusclassé parmi les modèles athérorésistants. Des désordres plasmatiques (hypertriglycéridémie et hypercholestérolémie) sont enregistrés. Les profils électrophorétiques des lipoprotéines affichent une baisse des molécules cardioprotectrices (HDL) et une augmentation des molécules d'athérogénicité (VLDL- LDL) témoignant d'un état proathérogène. L'observation sur plusieurs coupes histologiques des images d'agrégationssanguines traduit la réaction inflammatoire du processus athéroscléreux.Nous enregistrons également l'atteinte des composants conjonctifs (collagènes et lames élastiques) et cellulaires (cellules musculaires lisses) de la paroi aortique. Suite à ce protocole expérimental Rattus norvegicus a fini par céder aux effets délétères d'un régime riche en lipides et nous a permis d'apprécier certaines altérations athéroscléreuses de la paroi aortique.
Références
-Anand S S., "The value of studing gene-environment interactions in culturally divesrse populations", Rev. Can.Physiol.Pharmacol., 83, (2005), pp. 42-46.
-Kan-Merchant N., Penumetcha M., Meilhac O., Parthasarathy S., " Oxidized fatty acids promote atherosclerosis only in the presence of dietary cholesterol in low-density lipoprotein receptor Knockout mice", J.Nutr., 132, (2002), pp. 3256 – 3262.
-Han SN., Leka LS., Lichtenstein AH., Ausman LM., Schaefer EJ and Meydani SN., "Effect of hydrogenated and saturated, relative to polyunsaturated, fat on immune and inflammatory
responses of adults with moderate hypercholesterolemia", J.Lipid. Re., 43, (2002), pp. 445 – 452.
-Stocker R and Keaney J F., " Role of oxidative modifications in atherosclerosis", Physiol.Rev., 84, (2004), pp 1381- 1478.
-Schwab US., Ausman LM., Vogel S., Li Z., LammiKeefe CJ., Goldin BR., Ordovas JM., Schaefer EJ., Lichtenstein AH., " Dietary cholesterol increases the susceptibility of low density lipoprotein to oxidative modification", Atherosclerosis., 149, (2000), pp. 83-90.
-Hennig B., Toborek M and McClain CJ., " High- energy diets, fatty acids and endothelial cell function: implications for atherosclerosis", Am.Coll.Nutr., 2, (2001), pp. 97-105.
-Spiteller G., "Are lipid peroxidation processes induced by changes in the cell wall structure and how are these proceses connected with diseases? ", Med.Hyp., 60, (2003), pp. 69 – 83.
-Hadjiisky P., Renais J., Scebat L.," A comparative study of the arterial tissue metabolism atherosensitive and
atheroresistant species. Comparison between rabbit and rat aortas",Paroi Artérielle., 7, (1981), pp. 155 – 165.
-Hadjiisky P., Bourdillon MC., Grosgogeat Y., "Experimental models of atherosclerosis. Contribution limits and trends", Arch.Mal.Coeur. Vaiss., 84, (1991), pp.1593 – 15603.
-Kalwakami K., “ A rapid electrophoretic method for the detection of serum Lp(a) lipoprotein", Clin. Chem.Acta., 185, (1989), pp.147-156.
-Martoja R. et Martoja P.M., "Initiation aux techniques de l’histologie animale", Ed., Masson, 2ème édition, (1967), pp.296 – 300.
-Vidon C., Boucher P., Cachefo A., Peroni O., Diraison F and Beylot M.," Effects of isoinergetic highcarbohydrate compared with high-fatdiets on human cholesterol synthesis and expression of key regulatory genes of cholesterol metabolism", Am.J.
Clin.Nutr., 73, (2001), pp. 878 - 884.
-Andrééva-Gatéva., " Récepteurs activables par les proliférateurs des peroxisomes (PPAR) : leur implication dans le métabolisme des hydrates de carbone et des lipides", Ann. Biol. Clin., 61, (2003), pp. 295 – 303.
-Betteridge DJ.," What is oxidative stress?", Metabolisme., 49, (2000), pp. 3 – 8.
-Bird DA., Tangirala RK., Fruebis J., Steinberg D., Witztum JL and Palinski W.," Effect of probucol on LDL oxidation and atherosclerosis in LDL receptordeficient mice", J.Lip.Res., 9, (1998), pp. 1079 – 1090.
-Beckman JS., Beckman TW., Chen J., Marshall PA., Freeman BA.," Apparent hydroxy radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide",Porc.Natl.Acad. Sci. USA., 87, (1990), pp.1620 – 1624.
-Wieland H., Seidel D., Wiegand V., Kreuzer H., " Comparison of the lipoprotein profile with the results of coronary angiography", Atherosclerosis, 36, (1980), pp.269 – 280.
-Schaefer EJ., Foster DM., Jenkins LL., Lindgren FT., Berman M., Levy RI., Brewer HB., " The composition and metabolism of high density lipoprotein subfractions", Lipids, 14, (1979), pp. 511
– 522.
-Oorni K., Markku PO., Ala-Korpela M and Kovanen PT., "Aggregation, fusion,and vesicle formation of modified low density lipoprotein particles: molecular mechanisms and effects on matrix interactions. J. Lipid.Res., 41, (2000), pp. 1703 – 1714.
-Oorni K., Markku PO., Annila A and Kovanen PT., " Oxidation of low density lipiprotein particles decreases their ability to bind to human aortic proteoglycans", J.Biol.Chem., 272, (1997), pp.
– 21311.
-Markku OP., Oksjoki R., Oorni K., Kovanen PT., "Lipoprotein lipase in the arterial wall linking LDL to the arterial extracellular matrix and much more", Arterioscler.Thromb.Vasc.Biol., 22, (2002), pp. 211 – 217.
-Kontush A., Chantepie S., Chapman MJ., “ Small, dense HDL particles exert potent protection of atherogenic LDL against oxidative stress “Arterioscler.Thromb.Vasc.Biol., 23, (2003), pp.
– 1888.
-Calder PC., " Fat chance of immunomodulation", Immunol.Today., 19, (1998), pp. 244 – 247.
-Hadjiisky P., Renais J., Scebat L., " Histochimie et histo-enzymologie de l'épaississement diffus de l'intima aortique chez le lapin immunisé contre l'aorte de rat",Paroi Artérielle, 1, (1973), pp. 145 – 155.
-Zemplenyi T., Rosenstein AJ., "Arterial enzymes and their relation to atherosclerosis in pigeons", Exp.Mol.Pathol., 22, (1975), pp. 225 – 241.
-Hadjiisky P., Renais J., Scebat L., "Etude comparative des enzymes aortiques de rat (athérorésistant) et de lapin (athérosensible)", Bull.Ass.Anat., 58, (1974), pp. 571 – 584.
-Orbe J ; Rodriguez JA ; Arias R ; Belzunce M ; Nespereira B ; Pérez-Ilzarbe M ; Roncal C ; Paramo JA., "Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilization", Atherosclerosis, 167, (2003), pp. 45 – 53.
-Page IH., Brown HB., "Induced hypercholesterolemia and atherogenesis. Cir., 6, (1952), pp. 681 – 693.
-Scebat L., Renais J., Iris L., Lenegre J., " Athérosclérose expérimentale du rat. Etude anatomique des parois artérielles chez le rat hyperlipimique", Arch. Mal. Cœur., 56, (1963), pp.
– 55.
-Hess P., Staubli W., "The development of aortic lipoidosis in the rat : a correlative histochemical and electron microscopic study", Amer.J.Path., 43, (1963), pp. 301 – 335.
-Zemplenyi T., "Vascular enzymes and the relevance of their study to problems of atherogenesis", Med.Clin.N.Amer.,18, (1974), pp. 293 – 321.
-Dogru Abbasoglu S., Basaran-Kucukgergin C., Seckin
S., Oztezcan S., Solakoglu S ; Aykac-Toker G., Uysal M., "Cholesterol plus methionine feeding do not induce lipid peroxidation and atherosclerosis changes in the rat aorta", Int.J.Vitam.Nutr.Res., 72, 2(2002)., pp. 109 -113.
-Ozmen J., Bobryshev YV., Lord RSA and Ashwell KWS., "Identification of dendritic cells in aortic atherosclerotic lesions in rats with diet-induced hypercholesterolaemia. Histol.Histopathol., 17, (2002), pp. 223 – 237.
