HIGH LEVEL PRODUCTION OF THERMOSTABLE β-XYLANASE, CMC-ASE AND -GLUCOSIDASE BY AN ASPERGILLUS FUMIGATUS (FRESENIUS) ALBINO MUTANT STRAIN

Authors

  • N SARADOUNI Université de Tizi-Ouzou
  • M. J PENNINCKX Université Libre de Bruxelles,Brussels
  • O SALHI Université de Tizi-Ouzou

Keywords:

lignocellulosic materials, Carboxymethylcellulose, xylan, straw, cellulases, β-xylanases, thermophilic fungus, growth rate, submerged culture

Abstract

A local isolate of Aspergillus fumigatus (Fresenius) albino mutant obtained from rotten wood shavings, grew at 45°C on a solid medium containing cellulose as carbon source. This fungus can utilize a variety of lignocellulosic substrates, such as Kraft paper pulp, straw, microcrystalline cellulose and filter paper. The highest endoglucanase (CMC-ase), b-xylanase and b-glucosidase activities were obtained in shaken flasks at 45°C, with mainly oat straw as sole carbon source.

CMC-ase and b-xylanase activities showed the highest values (3.50 U/ml and 33 U/mI) at pH 4.0 and 5.0 respectively, and 70°C.

b-glucosidase activity was maximal at 65°C (43 U/ml ), CMC-ase  and b-xylanase enzymes were active in a large pH spectrum (3.0 to 8.0) and showed high thermostability at 60°C and above, especially for CMC-ase.

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Author Biographies

N SARADOUNI, Université de Tizi-Ouzou

Laboratoire de Microbiologie de I’URBAF, Institut de Biologie

M. J PENNINCKX, Université Libre de Bruxelles,Brussels

Unité de Physiologie et Ecologie Microbienne

O SALHI, Université de Tizi-Ouzou

Laboratoire de Microbiologie de I’URBAF, Institut de Biologie

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Published

1999-12-01

How to Cite

SARADOUNI, N., PENNINCKX, M. J., & SALHI, O. (1999). HIGH LEVEL PRODUCTION OF THERMOSTABLE β-XYLANASE, CMC-ASE AND -GLUCOSIDASE BY AN ASPERGILLUS FUMIGATUS (FRESENIUS) ALBINO MUTANT STRAIN. Sciences & Technology. A, Exactes Sciences, (12), 65–70. Retrieved from https://revue.umc.edu.dz/a/article/view/1639

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