REAL TIME PCR DIAGNOSIS OF LEISHMANASIS IN CONSTANTINE (ALGERIA)

Auteurs-es

  • I MIHOUBI Université Constantine 1
  • K FRAHTIA Université Constantine 1
  • N GASSEM CHU Constantine
  • S PICOT Hôpital de la Croix-Rousse, Hospices civils de Lyon, Lyon
  • F DEMONBRISON Hôpital de la Croix-Rousse, Hospices civils de Lyon, Lyon

Mots-clés :

Cutaneous leishmaniasis, Visceral leishmaniasis, Diagnosis, Real time PCR, Algeria

Résumé

Taking into account the re-emergence of leishmaniasis in the world, the geographic variability of its epidemiology and the growing numbers of travellers, a pilot study on the diagnosis of cutaneous leishmaniaasis and visceral leishmaniasis was undertaken in Constantine, one of the outbreak regions in eastern Algeria. A total of 143 cutaneous specimens and 108
bone marrow samples were collected on blotters and tested by real-time PCR. Results were compared with those of direct examination. Diagnosis, in CL, was positive for leishmaniasis in 81% of cases using PCR versus 48% of cases using microscopy. For VL, results were 26.9% for real time PCR versus 24% for microscopy exam. Real-time PCR showed a significant quantitative difference between patients for whommicroscopic diagnosis was positive and those for whom direct examination was negative. The results presented in thisstudy demonstrated the effectiveness and sensitivity of PCR in the diagnosis of cutaneous and visceral leishmaniasis from blotter specimens. This technique enabled in-field collection of specimens from each patient and provided prompt results. North- South cooperation based on the use of simple means for transmission of specimens for molecular diagnosis allowed creation of an effective partnership for daily diagnosis and promoted exchange between investigators in preparation for technology transfer

Bibliographies de l'auteur-e

I MIHOUBI, Université Constantine 1

Laboratoire de Biosystématique et Ecologie des Arthropodes

K FRAHTIA, Université Constantine 1

Laboratoire de Biosystématique et Ecologie des Arthropodes

N GASSEM, CHU Constantine

Laboratoire de Parasitologie –Mycologie Médicale

S PICOT, Hôpital de la Croix-Rousse, Hospices civils de Lyon, Lyon

Service de Paludisme, Parasites du sang et Mycologie Médicale

F DEMONBRISON, Hôpital de la Croix-Rousse, Hospices civils de Lyon, Lyon

Service de Paludisme, Parasites du sang et Mycologie Médicale

Références

- WHO (1990) Control of the Leishmaniases. Technical Report Series 793. WHO, Geneva, Switzerland, pp. 1–158.

- Desjeux P., Alvar J., Gradoni L., Gramiccia M., Medrano F.J., Deniau M. et al. Epidemiological analysis of 692 retrospective cases of Leishmania/HIV coinfections. WHO/LEISH, 1996 ; 39: 1-11

- Belazzoug S. Les leishmanioses en Algérie. A propos de

l’identification de 32 souches d’origine humaine et animale.

Leishmania. Taxonomie et phylogenèse. Applications écoépidémiologiquues (Coll. Int. CNRS/INSERM, 1984). Montpellier: IMEEE; 1986a. p. 397-400

- Harrat Z, Pratlong F, Belazzoug S, Dereure J, Deniau M, Rioux JA, et al. Leishmania infantum and Leishmania major in Algeria. Trans R Soc Trop Med Hyg 1996; 90:625-629.

- Belazzoug S, Ammar-Khodja A, Belkaid M, TabetDerraz O. Cutaneous leishmaniasis in northern Algeria [in French]. Bull Soc Pathol Exot Filiales 1985;78:615- 622.

- Izri MA, Belazzoug S. Phlebotomus (Larroussius) perfiliewi naturally infected with dermotropic Leishmania infantum at Tenes (Algeria). Trans R Soc Trop Med Hyg 1993;87:399.

- Benikhlef R, Harrat Z, Toudjine M, Djerbouh A, Bendali-Braham S, Belkaid M. Présence de Leishmania infantum MON-24 chez le chien. Méd Trop 2004;64:42-46.

- Belazzoug S. Isolation of Leishmania major Yakimoff & Schokhor, 1914 from Psammomys obesus Gretzschmar, 1828 (Rodentia: Gerbillidae), in Algeria. Trans R Soc Trop Med Hyg1983;77:876.

- Belazzoug S. Découverte d’un Meriones shawi (Rongeur, Gerbillidé) naturellement infesté par Leishmania dans le nouveau foyer de leishmaniose cutanée de K’sar Chellala (Algérie). Bull Soc Pathol Exot 1986;79:630-633.

- Izri MA, Belazzoug S, Pratlong F, Rioux JA. Isolation of Leishmania major in Phlebotomus papatasi in Biskra (Algeria). The end of an ecoepidemiological saga [in

French]. Ann Parasitol Hum Comp 1992; 67:31-32.

- Institut National de Santé Publique. Ministère de la Santé de la Population et de la Réforme Hospitalière. Direction de la Prévention. Relevé annuel des maladies à déclaration obligatoire Année 2005. Alger: Institut National de Santé Publique; 2005.

http://www.sante.dz/insp/rem.html [accessed 16 April 2009].

- Harrat Z., Addadi, K., Tabet Deraz O. - La leishmaniose viscérale en Algérie : recensement des cas de leishmaniose viscérale. Bull Soc Path Exo1992; 85: 296-301.

- Djerbouh A., Toudjine M., Djoudi M., Benikhlef R., Harrat Z. - La leishmaniose canine en Algérie : essai de traitement par l’allopurinol. Ann Méd Vét2005 ; 149 : 132-134.

- Izri A. ; Belazzoug S. – Diagnostic de laboratoire des leishmanioses rencontrées en Algérie. Revue francophone des laboratoires2007 ; Supplément au numéro 396,

- Mihoubi I., De Monbrison F., Romeuf N., Moulahem

T., Picot S. - Diagnostic délocalisé par PCR en temps réel de la leishmaniose cutanée dans le foyer de Constantine (Algérie). Med Trop2006; 66: 39-44

- Aoun K., Chouihi E., Amri F., Ben Alaya N,, Raies A,, Mary C,, Bourabtine A. Short Report: Contribution of Quantitative Real-Time Polymerase Chain Reaction to Follow-Up of Visceral Leishmaniasis Patients Treated with Meglumine Antimoniate. Am. J. Trop. Med. Hyg., 2009; 81(6): 1004-1006

- Mary C, Farault F, Droguoul MP, Xeridat B, Scleinitz N, Cuisenier, Dumon H., Reference values for Leishmania infantumparasitemia in different clinical presentations: quantitative polymerase chain reaction for therapeutic monitoring and patient follow-up. Am J Trop Med Hyg, 2006; 75: 858–863.

- Nicolas, L., Milon, G., Prina, E., 2002. Rapid differentiation of old world Leishmania species by LightCycler polymerase chain reaction and melting curve analysis. J. Microbiol. Methods. 51,295–299.

- Desjeux P. The increase in risk factors for leishmaniasis

worldwide. Trans R Soc Trop Med Hyg 2001;95:239-243.

- Mott KE, Nuttall I, Desjeux P, Cattand P. New geographical approaches to control of some parasitic zoonoses. Bull World Health Organ. 1995;73(2):247-57.

- Barchiche N..A., Madiou M. - Recrudescence des leishmanioses cutanées : à propos de 213 cas dans la wilaya de Tizi-Ouzou. Pathol. Biol.2009 ; 57 : 65–70

- Reithinger R., Dujardin J.C.- Molecular diagnosis of leishmaniasis: current status and future applications. J. Clin. Microbiol. 2007; 45:21–25.

- Alam M.Z., Abul Khair.Shamsuzzaman M., Kuhls K. , Scho¨nian G. - PCR diagnosis of visceral leishmaniasis in an endemic region, Mymensingh district, Bangladesh. Trop.

Med, International Health 2009; 14 (5) : 499–503

- Deborggraeve S., Boelaert M., Rija S,, De Doncker S., Dujardin J.C., Herdewijn P., Büscher P. - Diagnostic accuracy of a new Leishmania PCR for clinical visceral leishmaniasis in Nepal and its role in diagnosis of disease. Trop. Med, International Health 2008; 13 (11): 1378–1383

- Pandey K., Pandey B.D,, Mallik A.K., Kaneko O., Uemura H., Kanbara H., Yanagi T., Hirayama K. -Diagnosis of visceral leishmaniasis by polymerase chain reaction of DNA extracted fromGiemsa's solution-stained slides. Parasitol Res2010 ; 107:727–730

- Souza Leite R., de Almeida Ferreiraa S., Trindade Ituassub S., de Melob M.N., Silva Ribeiro de Andradea A. - PCR diagnosis of visceral leishmaniasis in asymptomatic dogs using conjunctival swab samples. Vet Parasitol 2010;170 : 201–206

- Fraga Leite T., Brustoloni Y., M., Lima R.B., Cavalheiros Dorva M.E., Oshiro E.T., Oliveira J., Lyrio De Oliveira A., Pirmez C. - Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children. Mem Inst Oswaldo Cruz, Rio de Janeiro 2010; 105(3): 310-313.

- Nasereddin A., Benssoussan- Hermano E., S chönian G., Baneth G., Jaffe C.L. - Molecular Diagnosis of Old World Cutaneous Leishmaniasis and Species Identification by Use of a Reverse Line Blot Hybridization Assay. J.Clin. Microbiol 2008; 2848–2855.

- Bastien P, Procop G.W., Reischl U. - Quantitative realtime PCR is not more sensitive than "conventional" PCR. J Clin Microbiol. 2008, 46(6):1897-1900.

- Quaresma P.F., Fonseca Murta S.M.,DE Castro Ferreira

E., Mariano DA Rocha-Lima A.V., Prates Xavier A.A., Ferreira Gontijo C.M. Molecular diagnosis of canine visceral leishmaniasis: Identification of Leishmania species by PCR-RFLP and quantification of parasite DNA by realtime PCR. Acta ropica2009; 111 ; 289-294.

- Schönian G, Kuhls K, Mauricio IL. Molecular approaches for a better understanding of the epidemiology and population genetics of Leishmania. Parasitology., 2010; 16:1-21

- Neffati A., Kallel K., Anene S., Kaouech E., Belhadj S.,

Ennigrou S., Chaker E. Choix des amorces : élément déterminant dans le diagnostic moléculaire de la leishmaniose cutanée. Pathol Biol, 2009, [Epub ahead of print]

- Silva E.S, Gontijo CMF, Melo MN - Contribution of molecular techniques to the epidemiology of neotropical Leishmania species. Trends in Parasitol 2005; 21 : 550-552.

- Frank D.T., Nasereddin A., Schnur L.F., Schonian G.,

Toz S.O., Jaffe C. L., Baneth G. Detection and Identification of Old World Leishmaniaby High Resolution Melt Analysis. Neglected Tropical diseases2010; 4: Issue 1, e581.

- De Monbrison F., Angei F., Staal A., Kaiser K., Picot S. - Simultaneous identification of the four human plasmodium species and quantification of plasmodium DNA load in human blood by real-time PCR. Trans R Soc Trop Med Hyg 2003; 97: 1- 4.

- Mihoubi I., Picot S., Hafirassou N.; Monbrison de F. - Cutaneous leishmaniasis caused by Leishmania tropica in Algeria. Trans R Soc Trop Med Hyg2008; 102, 1157-1159.

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Publié-e

2012-06-01

Comment citer

MIHOUBI, I., FRAHTIA, K., GASSEM, N., PICOT, S., & DEMONBRISON, F. (2012). REAL TIME PCR DIAGNOSIS OF LEISHMANASIS IN CONSTANTINE (ALGERIA). Sciences & Technologie. C, Biotechnologies, (35), 44–49. Consulté à l’adresse https://revue.umc.edu.dz/c/article/view/312

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