This work aims at evaluating the antioxydant activity taking count the content phenolics of the leave’s rosemary extracts (EBr, EAcOEt and En-BuOH), by the means of two methods: the β-carotene bleaching method and the DPPH assay. The analysis by HPLC revealed the presence of rutin and the catechin in the rosemary extracts. The results of β -
Carotene-linoleic acid assay have showed an inhibiting activity of oxidation of the linoleic acid for the whole of the extracts, the latter significantly remains lower than that of positive control BHT (P<0.001).The EAcOEt of rosemary showed the most important activity with a rate of inhibition equal to 79.34%. There is no significant correlation (R² = 0.313) between the total phenolic content and the antioxydant activity of rosemary extracts. The results of the DPPH assay showed that extracts: En-BuOH and EAcOEt of rosemary presented scavenging activity equal to 96.18%, 95.81%
respectively, which were even higher than that of the BHT (92.91%). The total polyphenolic content of rosemary extracts was correlated significantly (R² = 0.997) with their scavenging activity.


Rosemary; Phenolic compounds; flavonoids; HPLC; Antioxydant Activity

Texte intégral :



- Mata A T, Proenc C, Ferreira A R, Serralheiro M L M, Nogueira J M F and Araujo M E M. Antioxidant and antiacetylcholinesterase activities of five plants used as Portuguese food spices. Food Chem. Vol.103.

(2007).pp. 778-786.

- Atik bekkara F, Bousmaha L, Taleb bendiab S A, Boti J B and Casanova J. Composition chimique de l’huile essentielle de Rosmarinus officinalis L poussant à l’état spontané et cultivé de la région de Tlemcen. Biologie & Santé. Vol.7. (2007).pp. 6-11.

- Gonzalez-Trujano M E, Pena EI, Martinez A L, Moreno J, Guevara-Fefer P, Deciga-Campos M and Lopez-Munoz FJ. Evaluation of the antinociceptive effect of Rosmarinus officinalis L. using three different

experimental models in rodents. J Ethnopharmacol. Vol.111. (2007). pp. 476-482.

- Ozturk M, Aydogmus-Ozturk F, Duru M E and Topcu G. Antioxidant activity of stem and root extracts of Rhubarb [(Rheum ribes): An edible medicinal plant. Food Chem. Vol.103.(2007).pp. 623-630.

- Lebreton P, Jay M, and Voirin B. Sur l'analyse qualitative et quantitative des flavonoïdes. Chim. Anal. Fr. Vol.49. N°7. (1967). pp. 375-383.

- Wong C C, Li H B, Cheng KW and Chen F. A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing

antioxidant power assay. Food Chem. Vol.97.(2006).pp.705-711.

- Djeridane A, Yous M, Nadjemi B, Boutassouna D, Stocker P and Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem.Vol.97. (2006). pp. 654-660.

- Amarowicz R, Troszynska A and Shahidi F. Antioxidant activity of almond seed extract and its fractions. J food lipids. Vol.12.(2005. pp.344-358.

- Tepe B, Sokmen M, Akpulat H A and Sokmen A. Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chem.Vol.95.


- Lopes-Lutz D, S Alviano, D S, Alviano C P and Kolodziejczyk P. Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry. Vol.69 . (2008). pp.1732-

- Maataoui B S, Hmyene A and Hilali S. Activites antiradicalaires d’extraits de jus de fruits du figuier de barbarie [Opuntia ficus indica]. Lebanese Science Journal. Vol.7. (2006).pp .3-8.

- Gortzi O, Lalas S, Chinou I and Tsaknis J. Evaluation of the Antimicrobial and Antioxidant Activities of Origanum dictamnus Extracts before and after Encapsulation in Liposomes. Molecules. Vol.12.

(2007). pp. 932-945.

- Fecka I and Turek S. Determination of polyphenolic compounds in commercial herbal drugs and spices from Lamiaceae: thyme, wild thyme and sweet marjoram by chromatographic techniques. Food Chem. Vol.108.

(2008). pp. 1039-1053.

- Gomez-Caravaca A M, Gomez-Romero M, Arraez- Roman D, Segura-Carretero A and Fernandez-Gutierrez A. Advances in the analysis of phenolic compounds in products derived from bees. J Pharmaceutical and Biomedical Analysis. Vol.41. (2006). pp. 1220-1234.

-Erkan N, Ayranci G and Ayranci E. Antioxidant activities of rosemary [Rosmarinus Officinalis L.] extract, blackseed [Nigella sativa L.] essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem. Vol.110. (2008). pp. 76-82.

- Ho S C, Tsai T H., Tsai P J and Lin C C. Protective capacities of certain spices against peroxynitritemediated biomolecular damage. Food and Chemical Toxicology. Vol.46. (2008). pp. 920-928.

- Tsai P, Tsai T and Ho S. In vitro inhibitory effects of rosemary extracts on growth and glucosyltransferase activity of Streptococcus sobrinus. Food Chem. (2007).(in press).

Tawaha K, Alali F.Q, Gharaibeh M, Mohammad M and El-Elimat T. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chem. (2007). (in press).

- Falleh H, Ksouri R, Chaieb K, Karray-Bouraoui N, Trabelsi N, Boulaaba M and Abdelly C. Phenolic composition of Cynara cardunculus L. organs, and their biological activities .C. R. Biologies. Vol.331. (2008).

pp. 372-379.

- Podsedek A. Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT. Vol. 40. (2007). pp. 1-11.

- Maisuthisakul P, Pasuk S and Ritthiruangdej P. Relationship between antioxidant properties and chemical composition of some Thai plants. J Food Composition and Analysis. Vol.21. (2008). pp. 229-240.

- Merken H M and Beecher G R. Liquid chromatographic method for the separation and quantification of prominent flavonoid aglycones. J

Chromatography A. Vol.897.(2000).pp. 177-184.

- Justesen U and Knuthsen P. Composition of flavonoids in fresh herbs and calculation of flavonoid intake by use of herbs in traditional Danish dishes. Food Chemistry. Vol.73. (2001). pp. 245-250.

- Wojdylo A, Oszmianski J and Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem. Vol. 105. (2007). pp. 940-949.

- Le K, Chiu F and Ng K. Identification and quantification of antioxidants in Fructus lycii. Food Chem. (2007). [in press].

- Gachkar L, Yadegari D, Rezaei M B, Taghizadeh M, Astaneh SA and Rasooli I. Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food Chem. Vol.102. (2007).

pp. 898-904.

- Dorman H J D, Peltoketo A, Hiltunen R and Tikkanen M J. Characterisation of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs. Food Chem. Vol.83. (2003). pp. 255-262.

- Almela L, Sanchez-Munoz B, Fernandez-Lopez J A, Roca MJ and Rabe V. Liquid chromatograpic- mass spectrometric analysis of phenolics and free radical scavenging activity of rosemary extract from different

raw material. J Chromatography A. Vol.1120. (2006). pp. 221-229.

- Celiktas O Y, Hames Kocabas E E, Bedir E, Vardar Sukan F, Ozek T and Baser K H C. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and

seasonal variations. Food Chem. Vol.100. (2007). pp. 553-559.

- Kosar M, Dorman HJD and Hiltunen R. Effect of an acid treatment on the phytochemical and antioxidant characteristics of extracts from selected Lamiaceae species. Food Chem. Vol.91. (2005). pp. 525-533.

- Hayouni EA., Abedrabba M, Bouix M and Hamdi M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea

L. fruit extracts. Food Chem. (2007]). [in press].

- Ardestani A and Yazdanparast R. Antioxidant and free radical scavenging potential of Achillea santolina extracts. Food Chem. [2007] Vol.104: pp.21-29.

- Turkmen N, Velioglu Y S, Sari F and Polat G. Effect of Extraction Conditions on Measured Total Polyphenol Contents and Antioxidant and Antibacterial Activities of Black Tea. Molecules. Vol.12. (2007).pp. 484-496.

- Bourgou S, Ksouri R, Bellila A, Skandrani I, Falleh H and Marzouk B, Phenolic composition and biological activities of Tunisian Nigella sativa L. shoots and roots .C. R. Biologies. Vol. 331. (2008). pp. 48–55.

- Kartal N, Sokmen M, Tepe B, Daferera D, Polissiou M and Sokmen A. Investigation of the antioxidant properties of Ferula orientalis L.using a suitable extraction procedure. Food Chem. Vol.100. (2007). pp.



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