How to Cite

ELOUAR, I., & DJEKOUN, A. (2019). OXFORD NANOTECHNOLOGIE: A NEW ERA FOR GENOME SEQUENCING AND PRECISION DIAGNOCTICS. Journal of Sciences & Technology , 4(1). Retrieved from http://revue.umc.edu.dz/index.php/st/article/view/3077


The advancement made on sequencing technology over the last years has been impressive. However, a number of new instruments were commercialized, the most attractive and promising one was the MinIon from Oxford Nanopore technology, UK. It is a small USB device using the nanopore technology to sequence more than 100kbp of DNA single stranded in a short time without pre-amplification or optical steps. This review focusses on the use of the new sequencing technology to improve the molecular and the precision diagnostic. Herein, we expose the employment of MinIon device for characterization, monitoring and detection of mutations in infectious agents but also its application in precision diagnosis and mutation analysis in clinical oncology and immunologic research.


Bates M, Polepole P, Kapata N, Loose M., O’Grady J. 2016. Application of highly portable MinION nanopore sequencing technology for the monitoring of nosocomial tuberculosis infection. International journal of mycobactériologie. S24.

Boweden R., Davies RW., Heger A., et al. 2019. Sequencing of human genomes with nanopore technology. Nature Communications 10: 1869.

Brookes K, Patel T, Zapata-Erazo G, Barber I, Braae A, Clement N, Tamar Guetta-Baranes T, Chappell S. Morgan K. 2016. Identifying Polymorphisms in the Alzheimer's Related APP Gene Using the Minion Sequencer. Next Generat Sequenc & Applic. 3:1

Celaya, G., Perales-Calvo, J., Muga, A., Moro, F. & Rodriguez-Larrea, D. 2017. Label-free, multiplexed, single-molecule analysis of protein–dna complexes with nanopores. ACS nano 11, 5815–5825.

Chaisson M, Wilson R and Eichler E 2015. Genetic variation and the de novo assembly of human genomes. Nature Rev. Gen. 16 627–640

De Jong L, Cree1 S, Lattimore1 V, Wiggins G.A.R, Spurdle A S. 2017. Nanopore sequencing of full-length BRCA1 mRNA transcripts reveals co-occurrence of known exon skipping events. Breast Cancer Research. 19:127.

Deutekom HW., Kooter P., Greerligs J., et al. 2017. P177 NGS typing results using oxford nanopore sequencing: Can minion data be reliably used for HLA typing? Human Immunology. (78) supplement :190

Dondorp WJ and Wert MWR. 2013. The ‘thousand-dollar genome’: an ethical exploration. European Journal of Human Genetics. 21: S6–S26

Duan J., Zhuo S., Yao FJ., Zhang YN., Kan XF. 2016. A Single-molecule Mycobacterium Smegmatis Porin A Protein Nanopore Sensor for Host-Guest Chemistry. Chinese Journal of Analytical Chemistry. 44(12) : 1801-1807.

Euskirchen P, Bielle F, Labreche K, Kloosterman WP, Rosenberg S, Daniau M,

et al. 2017. Same-day genomic and epigenomic diagnosis of brain tumors using

real-time nanopore sequencing. Acta Neuropathol. 134:691-703.

Hoenen T. 2016. Sequencing of Ebola Virus Genomes Using Nanopore Technology. Bio Protoc. 5; 6(21): . doi:10.21769/BioProtoc.

Imai K. , Tamura K., Tanigaki T., Mari Takizawa M., et al. 2018. Whole Genome Sequencing of Influenza A and B Viruses With the MinION Sequencer in the Clinical Setting: A Pilot Study. Front Microbiol. 9: 2748

Kasianowicz J., Reiner JE , Robertson JWF, Henrickson SE, Rodrigues C, Krasilnikov OV. 2012. Detecting and Characterizing Individual Molecules with Single Nanopores in Nanopore-Based Technology in Methods in Molecular Biologie. Edited by Maria E. Gracheva, Springer edition. Pp264.

Ke and Shen, 2017. Molecular targeted therapy of cancer: The progress and future prospect. Frontiers in Laboratory Medicine. 1(2): 69-75

Kilianski A, Haas JL, Corriveau EJ, Alvin T Liem AT, Willis KL, Kadavy DR, Rosenzweig CN and Minot SS. 2015. Bacterial and viral identification and differentiation by amplicon sequencing on the MinION nanopore sequencer GigaScience. 4:12

Kullman L., Winterhalter M., Bezrukov SM. 2002. Transport of maltodextrins through maltoporin: a single-channel study. Biophys J. 82:803–812

Laver T, Harrison J, O’Neill PA, Moore K, Farbos A, Paszkiewicz K, Studholme DJ. 2015. Assessing the performance of the Oxford Nanopore Technologies MinION. Biomolecular Detection and Quantification 3: 1–8

Leggett RM., Clark MD. 2017. A world of opportunities with nanopore sequencing. Journal of Experimental Botany. 68 (20): 5419–5429.

Liu C., Xiao F., Hoisington-Lopez J., Lang K., Quenzel P., Duffy B., Mitra RD. 2018. Accurate Typing of Human Leukocyte Antigen Class I Genes by Oxford Nanopore Sequencing. J Mol Diagn. 20(4):428-435.

Ma Y, Shi N, Li M, Chen F, Niu H. 2015. Applications of Next-generation Sequencing in Systemic Autoimmune Diseases. Genomics Proteomics Bioinformatics. 13: 242–249

Matern B., Groenewe M., Slangen T., .Tilanus MG. vooter C. 2017. P095 ABO blood group typing with the Oxford nanopore minion. Human Immunology. 78 : Supplement : 112

Nerbert DW and Zhang G. 2019. Pharmacogenomics in Emery and Rimoin's Principles and Practice of Medical Genetics and Genomics. 7th edition. Academic press edition. Pp 572.

Minervini CF, Cumbo C, Orsini P, Anelli L, Zagaria A, Impera L, et al. 2017. Mutational analysis in BCR-ABL1 positive leukemia by deep sequencing based on nanopore MinION technology. Exp MolPathol. 103:33-7.

Norris AL., Workman REW., Fan Y, Eshleman JR., Timp W. 2016. Nanopore sequencing detects structural variants in cancer. Cancer Biology & Therapy.

, vol. 17, no. 3, 246–253

Ozsolak F. 2012. Third Generation Sequencing Techniques and Applications to Drug Discovery. Expert Opin Drug Discov. 7(3): 231–243.

Patel A, Belykh E, Miller EJ, George LL, Martirosyan NL, Byvaltsev VA. 2018. MinION rapid sequencing: Review of potential applications in neurosurgery. Surg Neurol Int. 9:157.

Purnell RF and Schmidt JJ. 2009. Discrimination of single base substitutions in a DNA strand immobilized in a biological nanopore. ACS Nano 3:2533–2538

Quick J., Grubaugh ND., Pullan ST., et al. 2017. Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples .Nature Protocols volume 12, pages 1261–1276

Quick J., Loman NJ., Duraffour et al., 2016. Real-time, portable genome sequencing for Ebola surveillance. Nature. 530 (11) : 228–232.

Runtuwene LJ, Tuda JSB, Mongan AE, Makalowski W, Martin C.

Frith MC, Imwong M, Srisutham S, Nguyen Thi LA, Nguyen Tuan N, Eshita Y, Maeda R, Yamagishi J. Suzuki Y. 2018. Nanopore sequencing of drugresistance-associated genes in malaria parasites, Plasmodium falciparum. SCienTiFiC REPORTS. 8:8286

Sauvage V, Boizeau L, Candotti D, Vandenbogaert M, Servant-Delmas A, Caro V, et al. 2018. Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples. PLoS ONE 13(3): e0194366. https://doi.org/10.1371/journal.pone.0194366

Steinbock LJ., Radenovic A. 2015. The emergence of nanopores in next generation sequencing. Nanotechnology 26 (7) : 074003 (5pp)

Suzuki A, Suzuki M, Mizushima-Sugano J, Frith MC. Makałowski W, Kohno T, Sugano S, Tsuchihara K. Suzuki Y. 2017. Sequencing and phasing cancer mutations in lung cancers using a long-read portable sequencer. DNA Research. 24(6): 585–596

Todd SM., Settlage RE., Lahmers KK., Slade DJ. 2018. Fusobacterium genomics using MinION and Illumina sequencing enables genome completion and correction. mSphere3:e00269-18.https://doi.org/10.1128/mSphere.00269-18.

Ton KNT, Cree1 SL, Gronert-Sum SJ, Tony R. Merriman TR.Lisa K. Stamp LK and Kennedy MK. 2018. Multiplexed Nanopore Sequencingof HLA-B Locus in Maori and Pacific Island Samples. Frontiers in Genetics. 9:152.

Tsimberidou AM., Eggermont AM., Schilsky RL. 2014. Precision cancer medicine: the future is now, only better. Am Soc Clin Oncol Educ Book. 2014:61-9. doi: 10.14694/EdBook_AM.2014.34.61

Wang H, Ettedgui J, Forstater J, Robertson JWF, Reiner JE, Zhang H, Chen S, Kasianowicz JJ. 2018a. Determining the Physical Properties of Molecules with Nanometer-Scale Pores. ACS Sens. 23;3(2):251-263

Wang S., Zhao Z., Haque F., Guo P. 2018. Engineering of protein nanopores for sequencing, chemical or protein sensing and disease diagnosis. Current Opinion in Biotechnology. (51):80-89.

Wang J., Moore NE., Deng YM., Eccles DA., Hall RJ. 2015. MinION nanopore sequencing of an influenza genome. Front Microbiol. 6: 766.

Wang Y , Yang O., Wang Z. 2015. The evolution of nanopore sequencing. 449:1-21.

Xing Ke and Lisong Shen. 2017. Molecular targeted therapy of cancer: The progress and future prospect. Frontiers in Laboratory Medicine. 1 :(2):69-75.


Download data is not yet available.