ETUDE FONCTIONNELLE DES SYSTEMES DE CAPTURE SYNAPTOSOMALE ET VESICULAIRE DE DOPAMINE
Keywords:
Amphétamine, Capture de dopamine, β carbolines, 6 hydroxy-dopamine, imipramine, synaptosome, vésiculeAbstract
L‘injection stéréotaxique unilatérale dans la substance noire de la 6 hydroxy-dopamine ( 6OH-DA) se traduit par une chute parallèle de la capture de dopamine tritiée au niveau synaptosomale de l'ordre de -70 % et dans les préparations vésiculaires de l'ordre de -69 %, comparées aux préparations issues de Rats non lésés. Ces résultats montrent que ces deux préparations synaptosomales et vésiculaires sont bien d'origine dopaminergique. D'autre part, une étude comparative de l'effet de quelques agents pharmacologiques (β carbolines, imipraminiques, amphétamine et les inhibiteurs purs de la capture synaptosomale) sur la capture synaptosomale et vésiculaire de la dopamine tritiée in-vitro, montre qu'ils agissent comme de puissants inhibiteurs. Nous montrons également que le transporteur vésiculaire diffère du transporteur synaptosomal par sa stéréospécificité et sa sensibilité aux agents pharmacologiques.
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References
- Randrup A., Munkad I., Fog R., Gerlach J., Molander L. "Possible roles of brain dopamine in mania and depression", IV Congress of the Polish Pharmacological Society, (M. Mazur and Lods eds.), (1973), p.35.
- Lee T., Seeman P., Tourtellote W.W, Farley I.J, Hornykiewicz O. "Binding of 3H-neuroleptics and 3H-apomorphine in Schizophrenic brains", Nature, 274, (1978), pp. 897-900.
- Nihon S., Yakurigaku Z., "Antiparkinsonnian agents applied in the treatment of Parkinson's disease or are under investigation for patients or model animals", Neuropsychopharmacology, 4, (1996), pp. 113-122.
- Budygin E.A., Gainetdinov R.R., Kilpatrick M.R., Rayevsky K.S., Mannisto P.T., Wightman R.M., "Effect of tolcapone, a catechol-O-methyltransferase inhibitor, on striatal dopaminergic transmission during blockade of dopamine uptake", Eur. J. Pharmacol. 370, (1999), pp. 125-131.
- Budygin E.A., Kilpatrick M., Gainetdinov R.R., Raevski K.S., Wightman R.M., "The effect of the dopamine neuronal reuptake inhibitor GBR 12909 on dopaminergic neurotransmission parameters in the rat striatum in vivo", Eksp. Klin. Pharmacol, 62(5), (1999), pp. 3-6.
- Budygin E.A., Kilpatrick M.R., Gaainetdinov R.R., Wightman R.M., "Correlation behavior and extracellular dopamine levels in rat striatum: comparaison of microdialys and fast cyclic voltammetry", Neurosci. Lett., 3, 281, (2000), pp. 9-12.
- Glowinski Iverson L.L., "Regional studies of - catechnolamines in the rat brain. I: The disposition of 3H - norepinephrine, 3H dopamine and 3H-DOPA in various regions of the brain", J. Neurochem., 13, (1966), pp. 655-669.
- Bonnet J.J., Lemasson M.H., Costentin J., "Simultaneous evaluation, by a double method of uptake inhibition and release of dopamine in synaptosomal preparation of rat striatum", Bichem. Pharmacol., 13 , (1984), pp. 2129-2135.
- Slimani M, Boucher D., Bonnet J.J., Kamenka J.-M., Costentin J., "Neurochemical and behavioral evidence for a central indirect dopaminergic agonist activity of GK13, a phencyclidine derivative". In sigma and Phencyclidine like Compounds as Molecular probes in Biology, eds.E F. Domino and J.M. Kamenka, NPP Books, (1987), pp. 511-520.
- Kantor L., Hewlett G.H., Gnegy M.E., "Enhanced amphetamine and K+ mediated dopamine release in rat striatum after reprated amphetamine: differential requirements for Ca2+ and calmodulin dependent phosphorylation and synaptic vesicles", J. Neurosci., 15, 19, (1999), pp. 3801-3809.
- Albe-Fessard D., Stutinsky F. and Libondan S., "Atlas stéréotaxique du diencéphale du rat blanc", Editions C.N.R.S (Paris).
- Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J., "Protein measurement witn th Folin phenol reagent", J. Biol. Chem., (1951), pp. 265-275.
- Slotkin T.A., Seidler F.C., Whitmore W.L., Salvaggio M. and Kirksey D.F., "Rat brain synaptic vesicles: uptake specificities of (3H) norepinephrine and (3H) serotonin in preparations from whole brain regions", J. Neurochem., 31, (1978), pp. 961-968.
- Naudin B., Bonnet J.J., Costentin J., "Acute l-Dopa pretreatment potentiats 6 hydroxy-dopamine induced toxic effects on nigrostriatal dopamine neurons in mice", Brain Research, 701, (1995), pp. 151-157.
- Miller DW., Abercrombie E.D., "Role of high-affinity dopamine uptake and impulse activity in the appeearance of extracellular dopamine in striatum after administration of exogenous L-Dopa: studies in intact and 6-hydroxydopamine rats", J. Neurochem., 72, (1999), pp. 1516-1522.
- Scherman D., Jaudon P., Henry J.P., "Characterisation of the monoamine carrier of chromaffin granule membrane by binding of (2-3H) dihydrotetrabenazine", Proc. Natl., Acad. Sci. U.S.A., 80, (1983), pp. 583-588.
- Grattini S., "Mechanisms of action anoretic agents", In: Medicaments et comportements alimentaires, Actualités de pharmacologie clinique, eds. P. Meyer, F. Chaouloff, J.C. Gilbert Rolland, 5, (1988), pp. 1-24
- Vaugeois J.M., "Caractéristiques de la liaison in vivo chez la souris d'un inhibiteur de la capture de dopamine le (3H) GBR 12783", thèse de Doctorat, Université de Rouen (France), (1992).
- Ferris R.M., and Tang F.L.M., "A comparison of the capacities of isomers of amphetamine, deoxypipradol and methylphenidate to inhibit the uptake of tritiated preparations of rat cerebral cortex, hypothalamus and striatum, and into adrenergic nerve of rabbits aorta", J. Pharmacol. Exp. Ther., 181, (1972), pp. 407-416.
- Schoemacker H. and Nickolson V.J., Dopamine uptake by striatal synaptosomes: a compartiment analysis", J. Neurochem., 41, (1983), pp. 684-690.
- Crespi D., Menniti T., Gobbi M., "Carrier dependent and Ca2+ dependent 5-HT and dopamine release p-choroamphetamine and (+) - fenfluramine", Br. J. Pharmacol., 121, (1997), pp. 1735-1743.
- Horn A.S., "Characteristics of dopamine uptake , In A.S Horn", J. Korf and B.H.C. Westerink (eds). The neurobilogy of dopamine, Academic press, New York , (1979), pp. 217-235.
- Dueterte Boucher. D, Kamenka J.-M. and Costentin J., "Comparison of the effects of the indirect dopamine agonists,GK 13, GBR 12783 and dexamphetamine on behavioural tests involving central catecholaminergic transmissions", Psychopharmacology, 101, (1990), pp. 344-353
- Kibourn M.R., Sherman P.S. and Pisani I., "In vivo binding of the dopamine uptake inhibitor (18F) 13119 in MPTP treated C57 BL /6 mice", Nucl. Med. Biol., 18 , (1992), pp. 803-806.
- Héron C., Costentin J., Bonnet J.J., Evidence that pure uptake inhibitors including cocaine interact slowly with the dopamine neuronal carrier", European Journal of Pharmacology, 264, (1994), pp. 391-398
- Daniels A.J., Gysling K., Arqueros L., "Intraneuronal site of action for imipramine in rat striatal slices", J. Neurochem., 35, (1980), p. 718-722
- Langer S.Z., Galzin A.M., Kamal L.A., "3H-imipramine is accumalated but not released from slices of the rabbit caudate and hypothalamus", J. Neurochem., 38, (1982), pp. 305-312
- Niznik H.B., Tyndale R.F., Sallee F.R., Gonzalez F.J. and Kalow W., "The dopamine uptake transporter and cytochrome b450IID1 in brain: resolution and identification of two distinct (3H) GBR 12935 binding proteins", Arch. Biochem. Biophs. 276, (1993), pp. 424-432.
- Wood M.D. and Wyllie M.G., "Subcellular site of action of imipramine in rodent brain", J. Neurochen ., 46, (1986), pp. 999-1005.
