LOCALISATION ULTRASTRUCTURALE DU CALCIUM ENDOGENE DANS DES ELECTRORECEPTEURS CUTANES DE POISSONS TELEOSTEENS
الكلمات المفتاحية:
calcium، électrorécepteurs، réticulum endoplasmique، vésicules synaptiques، gaine de myéline، terminaisons nerveusesالملخص
Le calcium a été identifié à l’échelle ultrastructurale par la méthode de Probst [1] dans trois électrorécepteurs cutanés spécifiques du système de la ligne latérale de poissons téléostéens : l'organe ampullaire et l'organe de type B ou Gymnarchomaste chez Gvmnarchus niloticus (Gymnarchidae) et l’organe tubéreux chez Apteronotus leptorhynchus (Gymnotidae). Dans ces organes sensoriels, le calcium endogène qui se présente sous forme de précipités denses aux électrons, est localisé dans des organites cytoplasmiques des cellules sensorielles et dans la fibre nerveuse afférente. Il est absent dans le "cleft" synaptique et peu concentré dans les cellules accessoires. Dans les cellules sensorielles, on trouve ces dépôts dans les vésicules synaptiques, surtout celles situées au voisinage des rubans synaptiques, dans les saccules du réticulum endoplasmique et entre les feuillets de la membrane nucléaire. Dans les mitochondries peu de dépôts sont présents et le diamètre des granulations est plus petit que celui observé dans les autres structures cellulaires. Dans la fibre nerveuse, de fines granulations sont présentes dans les saccules de réticulum, dans les neurotubules et les mitochondries. Les dépôts les plus importants sont situés entre les feuillets de la gaine de myéline.التنزيلات
المراجع
- Probst W., "Ultrastructural localization of calcium in the CNS of vertebrates", Histochem., 85, (1986), pp.231-239.
- Szerb, J. C., O’Regan P.A., "Effect of glutamate release from hippocampal slices induced by high K+ or by electrical stimulation: interaction with Ca++ concentrations", Journal of Neurochem., 44, (1985), pp.724-731.
- Wick S.M. and Hepler P.K., "Selective localization of intracellular Ca++ with potassium antimonate", J. Histochem. Cytochem., 31, (1982), pp.1190-1204.
- Van Reempts J., Borgers M. and Offner F., "Ultrastructural localization of calcium in the rat retina with a combined oxalate - pyroantimonate technique", Histochem. J., 14, (1982), pp.517-522.
- Mc Graw C.F., Somlyo A.V. and Blaustein M.P., "Localization of calcium in presynaptic nerve terminals", J. Cell Biol., 85, (1980), pp.228-241.
- Duce I.R. and Keen P., "Can neuronal smooth endoplasmic reticulum function as a calcium reservoir?", Neurosc., 3, (1978), pp.837-848.
- Silsbergeld E.K. and Costa J.L., "Synaptosomal Ca++ metabolism studied by electron microprobe analysis", Exp. Neurol., 63, (1979), pp.277-292.
- Ellisman M.H., Friedman P.L. and Hamilton W.J., "The localization of sodium and calcium to Schwann cell paranodal loops at nodes of Ranvier and calcium to compact myelin", J. Neurocytol., 9, (1980), pp.185-205.
- Chan S.Y., Ochs S. and Jersild R.A., "Localization of calcium in nerve fibers", J. Neurobiol., 15, (1984), pp.89-108.
- Joo F., Milhaly A. and Parducz A., "Visualization of actived synapses with the calcium inclusion technique", In: Jones D.G., ed., Current Topics in Research on Synapses, Alan R. Liss, New York, (1984), pp.119-163.
- Obara S. and Bennett M.V.L., "Mode of operation of ampullae of Lorenzini of skate", Raja. J. Gen. Physiol. 60, (1972), pp.534-557.
- Clusin W.T. and Bennett M.V.L., "Calcium activated conductance in skate electroreceptors". Current clamp experiments", J. Gen. Physiol., 69, (1977a), pp.121-143.
- Clusin W.T. and Bennett M.V.L. "Calcium activated conductance in skate electroreceptors. Voltage clamp experiments", J. Gen. Physiol., 69, (1977b), pp.145-182.
- Clusin W.T. and Bennett M.V.L., "The oscillatory responses of skate electroreceptors to small voltage stimuli", J. Gen. Physiol., 73, (1979a), pp.685-702.
- Clusin W.T. and Bennett M.V.L., "The ionic basis of oscillatory responses of skate receptors", J. Gen. Physiol., 73, (1979b), pp.703-723.
- Sugawara Y. and Obara S., "Damped oscillation in the ampullary electroreceptors of Plotosus involves Ca++ activated transient K+ conductance in the basal membrane of receptor cells", Brain Res., 302, (1984a), pp.171-175.
- Sugawara Y. and Obara S., "Ionic currents in the sensory epithelium examined in isolated electroreceptors of Plotosus under simulated in situ conditions", Brain Res., 302, (1984b), pp.176-179.
- Sugawara Y., "Two Calcium current components of the receptor current in the electroreceptors of marine catfish Plotosus", J. Gen. Physiol., 93, (1989), pp.365-380.
- Zakon H.H., "Tuning of newly generated electroreceptors", Neurosc., 10, (1984), p.193.
- Zipser B. and Bennett M.V.L., "Tedrodotoxin resistant electrically responses of receptor cells", Brain Res., 62, (1973), pp.253-259.
- Spurr A.R., "A low-viscosity epoxy resin embedding medium for electron microscopy", J. Ultrastruct. Res., 26, (1969), pp.31-43.
- Szabo T., "Sense organs of the lateral line system in some electric fish of the Gymnotidae, Gymnarchidae and Mormyridae", J. Morph., 117, (1965), pp.229-250.
- Szamier R.B. and Watchel A.W., "Special cutaneous receptors organs of fish: The ampullary organs of Eigenmania", J. Morphol., 128, (1969), pp.261-290.
- Mullinger A.M., "The orgazation of ampullary organ in the electric fish Gymnarchus niloticus", Tiss. and cell, 1, (1969) pp.31-52.
- Derbin C., "Les organes sensoriels des organes spécifiques des poissons électriques. Contribution à l’étude cytologique et cytochimique", Thèse de doctorat ès sciences, (1974). Paris.
- Freilhöfer D., Körtje K.H. and Rahman H., "Ultrastructural localization of endogenous calcium in the teleost retina", Histochem. J., 22, (1990), pp.63-72.
- Benshalom G. and Flück A., "Calcium-induced electron density in synaptic vesicles of afferent and efferent synapses on hair cells in the lateral line organ", Brain Res., 121, (1977) pp.173-178.
- Ripps H., Shakib M., Chappell R.L. and Mac Donald E.D., "Ultrastructural localization and X-ray analysis of calcium-induced electrondense deposits in the skate retina", Neurosc., 4, (1979), pp.1689-1703.
- Sugawara Y. and Kanaseki T., "A combines physiological and electron microscope study of the electroreceptor of marine catfish, Plotosus anguillaris (Lacépède), with special reference to the mecanism of afferent transmissions", J. Elect. Microsc., 25, (1976), pp.214-215.
- Burton P.R. and Laveri L.S., "The distribution, relationships to other organelles, and calcium sequestering ability of smooth endoplasmic reticulum in frog olfactory axons", J. Neurosci., 5, (1985), pp.3047-3060.
- Nicaise G., Gillot I., Julliard A.K., Keicher E., Blaineau S., Amsellem J., Meyran J.C., Hernandez-Nicaise M.L., Ciapa B. and Gleyza C., "X-ray microanalysis of calcium containing organelles in resin embedded tissue", Scanning Microsc., 3, (1989), pp.199-220.
- Ungar F., Piscopo I., Letizia J. and Holtzman E., "Uptake of calcium by the endoplasmic reticulum of the frog photoreceptor", J. Cell Biol., 98, (1984), pp.1645-1655.
- Akutsu Y. and Obara S., "Calcium dependent receptor potential electroreceptor of marine catfish", Proc. Jap. Acad., 50, (1974), pp.247-251.
- Denizot J.P., "Adenosine triphosphatases (ATPAse) on the sensory cell plasmic membranes of tuberous organs (electroreceptors) of Gnathonemus petersii (Mormyridae). Cytochemical study", Cell. Mol. Biol., 26, (1980), pp.653-662.
- Mata M., Staple J. and Fink D.J., "Ultrastructural distribution of Calcium within neurons. An oxalate pyroantimonate study", Histochemistry, 87, (1987), pp.339-349.
- Henkart M.P., Reese T.S. and Brindley F.J., "Endoplasmic reticulum sequesters calcium in squid giant axon", Science, 202, (1978), pp.1300-1302.
- Hillman D.E. and Llinas R., "Calcium-containing electron-dense structures in the axons of the squid giant synapsee, J. Cell Biol., 61, (1974), pp.146-155.
- Mata M., Alessi D. and Fink D.J., "S100 is preferentially distributed in myelin forming Schwann cell", J. Neurocytol., 19, (1990), pp.432-442.
- Djebar A.B., "Utilisation de la méthode de Probst (1986) pour la mise en évidence du calcium intracellulaire dans le système nerveux: remarques sur la méthodologie", Symposium national de microscopie électronique USTHB-Alger, (1999), p. 50.
- Gautron J., "Effet du calcium et de la stimulation sur les terminaisons nerveuses des jonctions nerf - électroplaque de la Torpille", Biol. Cell., 31, (1985), pp.31-44.
- Denizot J.P. and Libouban S., "New formation of sensory cells in the tuberous organ (electroreceptor) of Brienomyrus niger (Mormyridae) induced by transection of afferent nerve", Int. J. Devl. Neurosc., Vol. 3, 4, (1985), pp.323-330.
