EFFET ANTI-LISTERIADE BIFIDOBACTERIUM INFANTISISOLÉ A PARTIR DE SELLES DE NOURRISSON ALLAITÉ AU SEIN
Mots-clés :
Bactériocine, Bifidobacterium infantis, Effet anti-ListeriaRésumé
Une souche de Bifidobacterium infantisa été isolée à partir de selles de nourrisson âgé de deux mois allaité exclusivement au sein de l’hôpital Ben Boulaid de Blida. Cette souche est douée d’une
activité antimicrobienne à l’égard de Listeria monocytogenes. Une purification partielle de la bactériocine a été effectuée d’abord par précipitation des protéines au sulfate d’ammonium puis par un passage du surnageant brut actif en chromatographie à échangeur de cations avec une élution de la bactériocine par paliers à 0,1, 0,3 et 1M en NaCl. À ce stade de purification l'activité antimicrobienne
a été observée dans la fraction éluée à 0,1M en NaCl. Puis par un passagede la fraction active précédente en chromatographie en phase inversée. La bactériocine a été soumise à l'action de
plusieurs enzymes protéolytiques (pepsine, trypsine, pronase et proteinase K). Ces dernières altèrent complètement l'activité antagoniste de la bactériocine. L'effet dutraitement thermique a montré que cette substance reste stable pendant 30 min de 70°C. jusqu'à 90°C. L'activité inhibitrice de la bactériocine reste stable dans des variations du pH acide et neutre. Par contre elle décroît dans des conditions de pH basique. L’effet anti-Listeriade Bifidobacterium infantisa montré après 6 jours de conservation que le taux de décroissance de la souche cible est de97,05 % à 15°C. Ce taux est plus important (99,50 %) lorsque l’espèce cible est conservée à 4°C.
Références
- Pope C., Kim S. K., Marzo A., Williams K., Jiang J., Shen H. and Lefranc L. “Organ-Specific Regulation of the CD8 T Cell Response to Listeria monocytogenes”.Infection J. Immunol. 166, (2001), p.3402 - 3409.
- Servin A. L. “Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens”. FEMS Microbiol. Rev.(2004), 28, 4, p.405 - 409.
- Naghmouchi K., DriderD., KheadrE., Lacroix C., PrévostH. and FlissI. “Multiple characterizations of Listeria monocytogenessensitive and insensitive variants to divergicin M35, a new pediocin-like bacteriocin”. J. Appl. Microbiol. (2006), 100, p.29 - 34.
- Modler H. W. “Bifidogenic factors – sources, metabolism and applications”. Int. Dairy J.(1994), p.383 – 407.
- Tagg J. R., Dajani A. S. and Wannamaker L. W. “Bacteriocins of Gram positive bacteria”. Bacteriol. Rev.(1976), 40, 3, p.722 – 756.
- Thompson J.K., Collins M.A. and Mercer W.D. “Characterization of a proteinaceous antimicrobial produced by Lactobacillus helveticusCNRZ450”. J. Appl. Bacteriol. (1996), 80, 3, p.338 - 348.
- Nissen-Meyer J., Holo H., Havarstein L. S., Sletten K. and Nes I. F. “A novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides”.J. bacterial.174, 17, (1992), p.5686 - 5692.
- De Vuyst L. and Vandamme E.J. “Nisin a lantibiotic produced by Lactococcus lactisbusp lactis: properties. biosynthesis, fermentation and applications”. In: Bacteriocin of acid lactic bacteria: microbiology, genetics and applications. Blackie Academic and Professional, (1994), London.
- Mattick A.T.R. and Hirsch .A. “Further observations on an inhibitory substance (nisin) from lactic Streptococci”. Lancet., 2, (1947), p.5 - 7.
- Davey G.P. and Richardson B.C. “Purification and some propenies of diplocococin from Streptococcus cremoris 346”. Appl. Environ. Microbiol.41, (1981), p.84 - 89.
- Holo A., Silson O. and Nes I.F. “Lactococcin A, A new bacteriocin from Lactococcus Lacti subsp. cremoris: isolation and characterization of the protein and its gene”. J. Bacteriol. 173, (1991), p.3879 - 3887.
- Kashket E.R. “Bioemergetics of lactic and bacreria cytoplasmic pH and osmotolerence”. FEMS Microbiol. Rev.46, (1987), p.233 - 244.
- Bailey F.J. and Hurst A. “Preparation of highly active from of nisin from Sreptococcus lactis”. Can. J. Microbiol. 17, (1971), p.61 - 67.
- Hastings J.W., Sailer M., Johnson K., Roy K.L., Vederas J.C. and Stiles M.E. “Characterization of leucocin A-CAL 187 and cloning of the bacteriocin gene from Leuconosroc gelidum”. J. Bacterio1. 173, (1991), p.7491 - 7500.
- Nieto Lozano J.C., Nissen Mayer J., Sletten K., Pelaz C. and Nes I.F. “Purification and amino acid sequence of a bacteriocin produced by Pediococcus acidilactici”. J. Gen. Microbiol. 138, (1992), p.1985 - 1990.
- Muriana P.H. and Klaenhammer T.R. “Purification and partial characterization of lactacin F. a bacteriocin produced by Lactobacillus acidophilus 11088”. Appl. Environ. Microbiol.53, (1991), p.553 - 560.
- Piard J.C. and Desmazeaud M. “Inhibiting factors produced by lactic acid bacteria. 2. Bacteriocins and other antibacterial substances”. Le lait, 72, (1992), p.113 - 142.
- Daba H. Lacroix C., Huang J., Simard R.E. and Lemieux L. “Simple method of purification and sequencing of a bacteriocin produced by Pediococcus acidilactici UL 5”. J. Appl. Bacteriol.
, (1994), p.682 - 688.
- Yang R., Johnson M.C. and Ray B. “Novel method to extract large amounts of bacteriocins from lactic acid bacteria”. Appl. Environ. Microbiol.58, (1992), p.3355 - 3359.
- Bower C.K. and Daeschel M.A“Suppression of Listeria monocytogenes colonization following adsorption of nisin onto silica surfaces”. Appl. Environ. Microbiol. 61, (1995), p.992 - 997.
- Mulders J.W.M., Boerrigter U., Rollema H.S., Siezen R.I. and Vos V.M. “Identification and characterization of the lantibiotic nisin. a natural nisin variant”. Eur. J. Biochem.201, (1991), p.581 - 584.
- Mortvedt C.I., Nissen-Meyer J., Sletten K. and Nes
I. “Purification and amino acid sequence of lactocin S. a bacteriocin produced by Loctobacillus sake L45”. Appl. Environ. Microbiol.57, (1991), p.1829 - 1834.
- Holck A., Axelsson L., Birkeland S.E., Aukmst T. and Blom H. “Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb 706”. J. Gen. Microbiol.138, (1992), p.2715 - 2720.
- Meghrous J., Lacroix C., Bouksaim M., Lapointe G. and Sirnad R.E. “Genetic and biochemical characterization of nisin Z produced by Lactococcus lactis ssp. Lactis biovar. diacetylactisUL 719”.J. Appl. Microbiol.83, (1997), p.133 - 138.
- Suarez A.M., Azcona J.I., Rodriguez J.M., Sanz B. and Hemandez P.E. “One step purification of nisin A by immunoaffinity. Chromatography”. Appl. Environ. Microbiol.63, 12, (1997), p.4990 - 4992.
- Motlagh A.M., Bhunia A.K., Szostek F., Hansen T.R., Johnson M.C. and Ray B. “Nucleotide and amino acid sequence of pap-gene (pediocin AcH production) in Pediococcus acidilacticiH”. Lett. Appl. Microbiol. 15, 2, (1992), p.45 - 48.
- Benkerroum N., Oubel H., Zahar M., Dlia S. and Filali-Maltouf A. “Isolation of a bacteriocinproducing Lactococcus lactissubsp. lactisand application to control Listeria monocytogenesin Moroccan jben”. J. Appl. Microbiol.89, (2000), p.960 – 968.
- Davidson M.P. and Harrison M.A. “Resistance and
adaptation to food antimicrobials, Sanitizers, and other process controls”. Food technol.56, 11, (2002), p.69 - 78.
- Dicks L.M.T., Mellett F.D. and Hoffman L.C.“Use of bacteriocin-producing starter cultures of Lactobacillus plantarumand Lactobacillus curvatus in production of ostrich meat salami”. Meat Science, 66, (2004), p.703 - 708.
- Martinez R.C.R. and De Martinis E.C.P. “Antilisterial activity of a crude preparation of Lactobacillus sakei1 bacteriocin and its lack of influence on Listeria monocytogeneshaemolytic activity”. Food Control.16, (2005), p.429 - 433.
- Mercenier A., Pavan S. and Pot B. “Probiotics as
Biotherapeutic Agents: Present Knowledge and Future Prospects”. Current Pharmaceutical Design. 9, 2, (2003), p.175 - 191.
- Turchet P., Laurenzano M., Auboiron S. and Antoine J.M. “Effect of fermented milk containing the probiotic Lactobacillus caseiDN-114001 on winter infections in free-living elderly subjects: a randomised, controlled pilot study”. J. Nutr. Health Aging. 7, 2, (2003), p.75 - 77.
- Plummer S., Weaver M.A., Harris J. C., Dee P. and
Hunter J. “Clostridium difficilepilot study: effects of probiotic supplementation on the incidence of C. difficilediarrhoea”. Int. Microbiol.7, 1, (2004), p.59 - 62.
- Tursi A., Brandimarte G., Giorgetti G. M. and Elisei W. “Mesalazine and/or Lactobacillus caseiin Preventing Recurrence of Symptomatic Uncomplicated Diverticular Disease of the Colon: A Prospective, Randomized, Open-label Study”. J.
Clinical Gastroenterol. 40, 4, (2006), p.312 - 316.
- Wang Y.C., Yu R.C. and Chou C.C. “Viability of lactic acid bacteria and bifidobacteria in fermented soymilk after drying, subsequent rehydration and storage”. Int. J. Food Microbiol.93, 2, (2004), p.209 - 217.
- Rosenfeldt V, Michaelsen K. F., Jakobsen M., Larsen C. N., Moller P.L., Pedersen P., Tvede M., Weyrehter H., Valerius N. H. and Paerregaard A. “Effect of probiotic Lactobacillusstrains in young children hospitalized with acute diarrhea, Pediatr”. Infect. Dis. J.21, 5, (2002), p.411 - 416.
- Food and Drug Administration (FDA) “Nisin preparation: Affirmations of GRAS status as direct human food ingredient”. Food Drug Admin. Fed. Reg.(1988), 53, 11247.
- Delves-Broughton J. “Nisin and its uses as a food preservative”. Food Technol. 44, 11, (1990), p.100 - 117.
- Jung D.S., Bodyfelt F.W. and Daeschel M.A. “Influence of fat and emulsifiers on the efficacy of nisin in inhibiting Listeria monocytogenes in fluid milk”. J. Dairy Sci.75, (1992), p.387 - 393.
- Mulder H. and Walstra P. “The milk fat globule”. Ctr. Agric. Publ. Doc.(1974), Wageningen, The Netherlands.
