INFLUENCE OF SECONDARY METABOLIC PRODUCTS OF ASPERGILLUS FUMIGATUSON THE GERMINATION RATEOF SOME LENTIL TYPES
Mots-clés :
Secondary Metabolic, Aspergillus fumigatus, germination rate, lentil seedsRésumé
Our research purpose to study the effectof the metabolic by-products of the Aspergillus fumigatus fungus on the germination of some types of lentil seeds. Purification has been achieved with organic solvents prior to bar chromatography using an aluminium oxide bar Al2O3,fallowed by thin layer chromatography transported to Silica gel F254 plates. After drying, values of the circulation rate
(0.937-0.051)Rf for every resulting spotwere determined. Spots occur with different colours under UV radiation with short wave 254 nm and 365 nm for long waves and likewise in regular light. It
allowed determining ten close in their chemical structure. With the study of the impact of different concentrations from secondary metabolic issues secreted by Aspergillus fumigatuson lentil seed
germination: sp. I (Syrie), sp. II Large blonde (chili), sp. III Esculata lens (verte). The experiment was designed in sterilized sandy fields inside Petri.The minimal germination percentage rate for the lentil seeds (60.65%) was recorded at concentration 1 mg for Large blonde ( chili) and Escualata lens ( verte), whereas Syrieamounted to 70% at the same concentration. Furthermore, this rate was recorded for Esculata lens( verte)at concentration 100 µg and for LBC type at concentration 1 µg. That means resistance capacity in Syrieexceeds 100, 10 times the ELV and LBC capacity respectively. It should be pointed out that sensitivity in the lentil types started at concentration 0.25 ng and germination percentage was 92.95 and 95% for ELV, LBS, Syrierespectively.
Références
- Abrason, D.N.;A. Simber And J.T. Mills(1983).Mycotoxin formation in barley stored at 16 and 20% moisture in Manitoba.
Cereal.Chem.60(5):350 - 355
- ATALAH ,M.M.; M.M. FAHIM And Y.A. ABDOU (1979) .International phytopath ,conference ,2 ,Cairo ,21-24 March 1979:745 -756.
- Atallah ,M.M. (1983) International mycotoxin Conference 1 Cairo , 19 th March (Abst).
- Bhutta ,A. R; And Ahmed S. (1989). Seed-brone fungal
pathogens of maize in Pakistan . J. of Sci . Industrially Research. 32: 107-109.
- Bottamley, R. A. ; Christensen, C. M. And W. F. Geddes. (1952). Grain storage stadies 10 : The influence of aeration time, and moisture content on fat acidity, non reducing sugar, and molde flora of stored yellow corn. Cereal. Chem. 29 :53-64.
- Botton ,B.;Breton , A. ;Fevre,M. Guy ,Ph. Larpent ,J. ;
P. Veau , (1990). Moisissures utiles et nuisibles importance industrielle . 2 ° Edition Massan ,Paris , New-york , Barcelonne ,Millon , Mexico , Saopawlo .
- Christensen ,C. M. (1964). Effect of moisture content
and lenght of storage period upon germination percentage of seeds of corn, wheat, and barley free storage fungi. Phytopathology ,54:1464-1466.
- Dehimat ,L. (1990). Etude des mycotoxines de moisissures contaminant les céréales et légumes secs stockés dans l’Est Algérien . Thèse de magister ISN . Univ. Constantine .
- DORTHY,E.T. And M.C. CHRISTENSEN (1986) . Aspergillus fumigatus a new species in the Aspergillus ochraceusgroups . Mycologia, 78(3):475-477.
- Hess , W. , And Stocks , D.C. (1969) . Surface characterizes of Aspergillusconidia . Mycologia, 61:560 -571 .
- Kirksy ,J. W. And R. J. Colle (1977) . Screening for
toxin production fungi . Mycopathologia et Mycologia Application , 54 : 292 1- 29 .
- Moreau , F. (1985). Les champignons . Physiologie .
Morphologie. Développement et Systématique , P:1360-136
- Moreau ,F. (1968 ) . Moisissures toxiques dans
l’alimentation . Encyclopédie Mycologique XXXV , Paris .
- Moreneau ,M.E. and M.C. Christensen (1970 ). Effect
of moisture and fungi on the viability of stored maize. Reveta. Lat. An. Microbiol.12 : 115 - 121 .
- Nahdi, S. ; Nurath,M. ; Batoul,H. And V. Nagamani
.(1982). Aflatoxin B1 contamination and seed mycoflora of gran (Cicer arietinum L.) during storage . Indian J. Bot. 5(2) : 196 – 198.
- Pitt ,J. I; Et A.D. Hocking (1985) . Fungi and food
silage . Academic Press Sydney.
- Robert , A. , Samson Ellem,S . ; Hoekatra And A. N .
Cenni Vanoorschat (1981) Introduction to fungi control . Bureau Voorchime , Cultures Institutes of the Royal Netherland Academy of Arts and Sciences
- Spilsbury, J. F. And Wilkinson, S. (1961). The isolation of festuclavine and two new clavine alkaloids from Aspergillus fumigatus Fress .J.Chem. Soc . 2085 - 2091.
