OXIDATIVE STRESS IN STREPTOZOTOCIN-INDUCED EXPERIMENTAL DIABETES IN RATS IS ASSOCIATED WITH CHANGES OF ANTIOXIDANT STATUS OF HEART TISSUE.
Mots-clés :
Streptozotocin, Antioxidant, Hyperglycaemia, Diabetes, Heart, RatRésumé
The present study was designed to evaluate the oxidative stress-related parameters in streptozotocininduced diabetes in rats. After 2, 3, 6 and 12 weeks of hyperglycaemia the enzymatic parameters were measured in heart tissue of diabetic and control groups. Superoxide dismutase, glutathione peroxidase and the glucose-6-phosphate dehydrogenase activities significantly increased at all time intervals.Catalase activity increased initially (after 2 and 3 weeks), decreased after 6 weeks and increased again at the 12th week of the experiment. Glutathione reductase activity increased initially (at 2nd week), decreased below control level after 3 weeks, and then increased again. Ascorbic acid concentration
decreased after 3 and 6 weeks, and increased at the 6th and 12th weeks. The level of lipid peroxidation products was reduced after 2, 3 and 6 weeks of the experiment. After 12 weeks it was significantly elevated. These data suggest that chronic hyperglycaemia induces oxidative stress in the heart but the
defense mechanisms in the heart tissue are fairly efficacious against oxidative injury.
Références
- Aebi H. Catalase in vitro. Methods Enzymol. 105 (1984) 121–6.
- Akkus I, Kalak S., Vural H., Caglayan O., Menekse E., Can G., Durmus B. Leukocyte lipid peroxidation, superoxide dismutase, glutathione peroxidase and serum and leukocyte vitamin C levels of patients with type II diabetes mellitus. ClinChim Acta. 244 (1996) 221–7.
- Bonnefont-Rousselot D., Bastard J.P., Delattre J. Consequences of the diabetic status on the oxidant/antioxidant balance. Diabetes Metab. 26 (2000) 163–76.
- Doi K., Sawada F., Toda G., Yamachika S., Seto S., Urata Y., Sakata N., Tanigchi N., Kondo T., Yano K.Alteration of antioxidants during the progression of heart disease in streptozotocin-induced diabetic rats. Free Radic Res. 34 (2001) 251–61.
- Ellman G.L. Tissue sulfhydryl groups. Arch Biochem. Biophys. 82 (1959) 70–7.
- Giugliano D., Ceriello A. Oxidative stress and diabetic vascular complications. Diabetes Care. 19 (1996) 257–67.
- Inouye M., Hashimoto H., Mio T., Sumino K. Levels of lipid peroxidation product and glycated hemoglobin A1c in the erythrocytes of diabetic patients. Clin Chim Acta. 276 (1998) 163–72.
- Inouye M., Mio T., Sumino K. Link between glycation and lipooxidation in red blood cells in diabetes. Clin Chim Acta. 285 (1999) 35–44.
- Jakus V. The role of free radicals, oxidative stress and antioxidant systems in diabetic vascular disease. Bratisl Lek Listy. 101 (2000) 541–51.
- Julius D, Militante J.B., Lombardini B., Schaffer S. W.The role of taurine in the pathogenesis of the cardiomyopathy of insulin-dependent diabetes mellitus. Cardiovascular Research. 46 (2000) 393–402.
- Kakkar R., Mantha S.V., Kalra J., Prasad K.Time course study of oxidative stress in aorta and heart of diabetic rat.Clin Sci. 91 (1996) 441–8.
- Kinalski M., Sledzeiwski A., Telejko B., Zarzycki W., Kinalska I. Antioxidant therapy and streptozotocininduced diabetes in pregnant rats. Acta Diabetol. 36 (1999) 113-117.
- Kowluru R.A., Engerman R.L., Kern T.S. Diabetesinduced metabolic abnormalities in myocardium: effect of antioxidant therapy. Free Radic Res. 32 (2000) 67–74.
- Kyaw A. A simple colorimetric method for ascorbic acid determination in blood plasma. Clin Chim Acta. 86 (1978)153–67.
- Lowry O.H., Rosebrough N.J.; Farr A.L..; Randall R.J. Protein measurement with Folin phenol reagent. J Biol Chem.193 (1951) 265–7.
- Löhr G.W., Waller H.D. Glucose-6-phosphate dehydrogenase, in: methods of enzymatic analysis, ed. By Bergmayer, H.U. Acad. Press, Newyork (1965) 744-751.
- Mak D.H., Ip S.P, Poon M., Ko K.M. Alterations in tissue glutathione antioxidant system in streptozotocininduced diabetic rats. Mol Cell Biochem. 162 (1996) 153–8.
- Matkovics B., Sasvari M., Kotorman M., Varga I.S., Hai D.Q., Varga C . Further prove on oxidative stress in alloxan diabetic rat tissues. Acta Physiol Hung 85 (1997/1998) 183–92.
- Opoka-Winiarska V. Impaired metabolism and effects of vitamin supplementation on diabetes. Diabetol Pol. 7 (2001) 208–11.
- Melvin R., Suresh C. Islet redox stress: the manifold toxicities of insulin resistance, metabolic syndrome and amylin derived islet amyloid in type 2 diabetes mellitus.JOP. J Pancreas 3 (4) (2002) 86-108.
- Parinandi N.L, Thompson E., Schmid H.H.O. Diabetic heart and kidney exhibit increased resistance to lipid peroxidation. Biochim Biophys Acta. 1047 (1990) 63–9.
- Pèrez C., Canal J.R., Torres M.D. Experimental diabetes treated with ficus carica extract : effect on oxidative stress parameters. Acta Diabetol.40 (2003) 3-8.
- Stefek M., Sotnikova R., Okruhlicova L., Volkovova K., Kucharska J., Gajdosik A., Gajdosikova A., Michalova D., Hozova R., Tribulova N., Gvozdjakova A.. Effect of dietary supplementation with the pyridoindole antioxidant stobadine on antioxidant state and ultrastructure of diabetic rat myocardium. Acta Diabetol. 37 (2000)111–7.
- Sun F., Iwaguchi K., Shudo R., Nagaki Y., Tanaka K., Ikeda K;, Tokumaru S., Kojo S. Change in tissue concentrations of lipid hydroperoxides, vitamin C and vitamin E in rats with streptozotocin-induced diabetes. Clin Sci. 96 (1999)185–90.
- Sydow K., Münzel T. Diabetes mellitus, oxidative stress and endothelial dysfunction. International Congress Series 1253 (2003) 125– 138.
- R. Tatsuki, K. Satoh, A. Yamamoto, K. Hoshi, K. Ichihara. Lipid peroxidation in the pancreas and other organs in streptozotocin diabetic rats. Jpn J Pharmacol. 75 (1997) 267–73.
- Yadav P, Sarkar S., Bhatnagar D. Lipid peroxidation and antioxidant enzymes in erythrocytes and tissues in aged diabetic rats. Indian J Exp Biol 35 (1997a) 389–92.
- Yadav P., Sarkar S., Bhatnagar D. Action of Capparis decidua against alloxan-induced oxidative stress and diabetes in rat tissues. Pharmacol Res. 36 (1997b) 221–8.
- West I.C. Radicals and oxidative stress in diabetes. Diabete Med. 17 (2000) 171–80.
- Ishii H., Koya D., King J.L.Protein kinase C activation and its role in the development of vascular Complications in diabetes mellitus, J. Mol. Med. 76 (1) (1998) 21–31.
- Esterbauer H., Schaur R.J., Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free radical Biology and medicine. 11, (1991), 81-128.
- Wajcman H., Galactéros F.Le déficit en glucose-6 phosphate déshydrogénase : protection contre le paludisme et risque d’accidents hémolytiques. C.R.Biologie. 327 (2004) 711–720.
- Kirkman H.N., Rolfo M., Ferrari A.M., Gaetani G.F. Mechanisms of protection of catalase by NADPH, J. Biol. Chem. 274, (1999), 13908–13914.
- Trinder P. Determination of glucose in blood using glucose oxidase with alternative oxygen acceptor. Ann. Clin. Biochem. 6 (24),(1969).
