ASSESSMENT OF THE CHANGES IN SOME DIAGNOSTICS ENZYMES AND OTHER PARAMETERS IN Wistar albinoRATS TREATED WITH PESTICIDES DURING GESTATION
Mots-clés :
organophosphorus pesticides, hepatotoxicity, teratogenic, liver function, pregnant ratesRésumé
The present work investigates the serum enzymes activities (LDH, ALP, AST, ALT, CPK) and the level of cholesterol, glucose, triglycerides, bilirubin, creatinine, and the total protein in female rats treated with pesticides; Chlorpyrifos-ethyl and Phosalone. Pregnant rats were divided into eleven groups containing 10 individuals each, and classified intothree categories were treated orally (from the 6th to the 15th day of gestation) with different doses (1/5, 1/4, 1/3, 1/2, 2/3 LD50) of both pesticides .Animals were dissected in the 19th day of gestation, and then blood samples were collected for biochemical study.
The obtained results showed that the liver is the target organ for pesticides toxicities, as indicated by the perturbations in its functions. Thus, a significant elevation in serum AST, ALT and ALP activities has been observed, while their liver activities were highly decreased. Serum cholesterol and triglycerides
levels were also decreased significantly. However, after treatment with different doses of both pesticides, the liver wet weights were highly increased, whereas, the foetus weights were significantly decreased. Generally, the degrees of observed variations were found to be dose dependent.
Références
- Simic B and Kniewald J.(1980). Effect of pesticides on
reproductive system. Acta pharmaceutica jugoslavia 30, 59-72.
- Deskin R., Rosenstein, N. Rogers and B. Westbrook (1979)
.Parathion toxicity in prenatal rats exposed in utero . toxicology
letters 3, 11-14.
- Shih, T.M., Cowan, F. and Koviak, T. (1988). Relationship
between suman-induced lethality and depression of tissue
cholinesterase (ChE) activity Fect. Am. Soc. Exp. Biol. J. 2,1791.
-Tezak Z, B. Simic and J. Kniewald (1992). Effect of
pesticides on oestradiol-receptor complex formation in rat uterus
cytosol . Fd. Chem. Toxic. , vol 30, N°10, 879-85.
-Mattsson J.L,Wilmer J.W., Shankar M.R., Berdasco
N.M.,Crissman J.W.,Maurissen J.P. and Bond D.M. (1996).
Single-dose and 13week repeated-dose neurotoxicity screenings
studies of chlopyrifos. Food.Chem. Toxicol. 34:393-405.
- Muto M.A. Lobelle F. Jr, Bidanset J.H. Wurpel J.N. (1992).
Embryo toxicity and neuro toxicity in rats associated with prenatal
exposure to DURSBAN.Vet. Hum. Toxicol. 34: 498-501.
-Dam K., Garcia S.J., Seidler F.J., Slotkin T.A.
(1999).Neonatal chlorpyrifos exposure alters synaptic
development and neuronal activity in cholinergic and
catecholaminergic pathways. Brain Res. Dev. Brain. Res. 116: 9-20.
]- Gupta J., Datta C., Sarkar A., Sengupta D. (1992). Effect of
Malathion on antioxidant defense system in human fetus-an in
vitro study. Ind. J. Exp. Biol. 30: 352-354.
-Hunter D.L., Lassiter T.L., Padilla S. (1999).Gestational
exposure to chlorpyrifos: comparative distribution of
trichloropyridinol in the fetus and dam. Toxicol. Appl. Pharmacol.
: 16-23.
-Nieves Tormo, Juan Raman Gimeno, Miguel Angel Sogorb,
Nuria Diaz-Alejo and Engenio Vilanova (1993). Soluble and
particulate organophosphorus Neuropathy target esterase in brain
and sciatic nerve of the hen cat, rat and chick. Journal of
Neurochemistry vol 61, N°6, 2165-68.
- Donald M. Maxwell, Karen M. Brecht, Bhupendra P.
Doctor and Alan D. Wolfe (1993). Comparison of antidote
protection against Soman by pyridostigmine, HI-Gand acetyl
cholinesterase. The journal of pyharmacology and experimental
therapentics vol 264, N°3. 1085-89.
- Dikshith T.S.Tandon S.K., Datta K.K., Gupta P.K. and
Behari,J.D.(1978).Comparative response of male rats to parathesis
and lindane: histopathologicaland biochemical studies.
Environmental research 17, 1-9.
]- GomesJ,Dawodu AH, Liyod O,Anilal SV(1999). Hepatic
injury and disturb amino acid metabolism in mice following
prolonged exposure to organ phosphorus pesticides. Vet. Hum.
Toxicol. 34:498-501.
- Bagchi D, Bagchi M, hassoun EA, and Stohs SJ (1995). In
vitro and in vivo generation ofreactive oxygen species, DNA
damage and lactate dehydrogenase leakage by selected
pesticides.Toxicology, 104:129-140.
- Dam K, Seidler Fj and Slotkin TA. (1998).Developmental
neurotoxicity of chlorpyrifos: delayed targeting of DNA synthesis
after repeated administration. Brain Res.Dev.Brain Res.;108 :39-45.
- Roy TS, Andrews JE,Seider FJ and Slotkin TA.(1998).
Chlorpyrifos elicits mitotic abnormalities and apoptosis in
neuroepithelium of cultured rat embryos.Teratology , 58: 62 – 86.
- Song X, Castrucci AML and Lamy-Freund MT, (1998).
Modeling the developmental neurotoxicity of chlorpyrifos in vitro:
macromolecule synthesis in PC12 cells . Toxicol. And Appl.
Pharmacol. 151:182-191.
- Chanda SM and Pope CN.(1996). Neurochemical and
neurobehavioral effects of repeated gestational exposure to
chlorpyrifos in maternal and developing rats.
Pharmacol.Biochem.Behav. 53:771-776.
- Witney KD, Seidler FJ and Slotkin TA (1995) .
Developmental neurotoxicity of chlorpyrifos : cellular
mechanism.Toxicol. And Appl. Pharmacol. 134:53-62.
- Wootton, I.D.P (1964).Micronalysis in medical
biochemistry.London: churchill.
[ 21]- Lowry, O.H. Rosebrough, N.J., Farr A.L. and Randal, R.J.
(1951). Protein measurement with folin-phenol reagent.J.
Biochem. 193,265.
- Bligh E.G. And W.J. Dyer (1959). A rapid method of total
lipid extraction and purification . can.J. Biochem. Physiol., 37-911.
- Van Handel E. and D.B. Zilversmit, (1957). Micro method
for determination of serum triglycerides. J. Lab. Clin. Med., 50,
[ 24]- Rice, E.W. (1970)Standared Method in clinical chemistry.
R.P. Mac Donald (Ed), Academie press, New York, 6, 215.
[ 25]- Rizk, G.A., Ali M.A., Sheta I.B. and Seleim Z.M. (1990).
Effect of acyl urea derivatives on the liver of mouse Mus
musculus.J. Egypt toxicol., 6: 73-88
- Seleim, Z.M. 1988.Histological and biochemical studies of
some acyl urea derivatives on white mice.Ph.D. Thesis, Pesticide,
facility of Agric. Minia Univ. PP. 242.
[ 27]- Esia, Amal A. And Selium Z.M. (1992).Effect of
Alphamethrinon hepatorenal function on mall [albino mice Mus
musculus J. Egypt. Soc. Txical. 9: 45-49.
- Kadota, T. Okuna, Kohdo, H. and Mtyamoto J. (1976).
Mammalians toxicological study ofpermethin, 3 phenoxy benzyl
(±) astrans –2,2- dimethyl-3-(2, 2 dichlorvinyl) –cyclopropane –1-
carboxylate. Botyukgoku, 41: 143-151.
- Abston P.A. and Yarbrough J.D (1976).The in vivo effects
of Mirex on soluble hepatic enzymes in the rat. Pesti. Biochem.
Physiol. , 6 (1): 192-199.
[ 30]- Robinson, K.M. and Yarbrough J.D. (1978). Liver response
to oral administration of Mirex inrates. Pest. Biochem. Physiol.8
(1): 65-72.
-Pradeep Bhatnagar and Nirmala Jain, (1986).
Morphofunctional changes in the liver of male mice after chronic
treatment with Phosphamidon. Bull.Environ. Contam. Toxicol.
, 767 – 773.
- Young M.F., Trombetta, L.D.and carson, S., (1986).Effect
of diflubenzuron on the mouse liver. J. Appl. Toxicol. 6 (5): 343-348.
-Quest,J.A., Copley,M.,P., Hamernik,K.L., Rinde,E.,
Fisher,B., Engler,R., Burnam,W.L. and Fenner-Crisp,P.A. (1990).
Evaluation of the carcinogenic potential of pesticides. - Reg.
Toxicol.12, 117-126
-Chang, J.C.,Pavkov,K.L., Campbell,W.R.,andWyand,D.S.
(1991) 90-day oral toxicity study with methidathion in beagle
dogs . Toxicologist 11,159.
[ 35]- Kiran, R.M., Bansal M. and Bansal R.C. (1988). Effect of
carbamate on some enzymes of rat liver and kidney. pesticides,
, (3), 8-10.
[ 36]- Uppal, R.P. and Ahmed A.(1977). Blood cholinesterase and
serum transaminase in Malathion toxicity in buffalo calves Ind. J.
Anim. Sci. 47 (10): 636-639
-Abd Alla, E.A.M.; Ahmed,N.S.;Hassan M.N.A. and Fouzy,
A.S.M.(1996). Malathion administration and its effect on some
liver and kidney function.Proc.of 3
rd
CTOX6DC,19-23Nov.,1995,Cairo,Vol.III,61-68.
-Abdel-Raheem, K. El-Elaimy I., El-Mossallamy N. And
Sherief N. (1984). Biochemical responses to induced intoxication
with insecticides. Effect of acute and repeated administration of
pyrethroid.Proc. Zool-Soc. A.R. Egypt. 13: 141-156.
- Saleh,N.A. and Mahmoud M.I.(1996) . Effect of sub lethal
doses of fenvealerate on certain biochemical parameters of cotton
leaf worm and male rats.3
rd
CTOX6DC,19-23 Nov.,1995
Proc.Vol.III:15-26.
-Seleim,Z.M . (1992).Hematological and biochemical studies
on white male rats exposed to two benzoylphenyl urea
compounds. J.Egyp. Soc. Toxicol. 9:75-80.
- Seleim,Z.M .(1995). Abamectin intoxication in rats and
effect on liver functions. J.Egyp. Soc.Toxicol.14:113-118.
- Terada,M.,Mizuhashi, F. Murata, K. Inoue,H. and
Tomita,T.(1998). Mepanipyrim inducesfatty liver in rats but not
mice or dogs. J. Toxicol. Sci.23;223-234
- Terada,M.,Mizuhashi, F. Murata, K. and Tomita,T.(1998).
Effect of Mepanipyrim on lipid metabolism in rats. J. Toxicol.
Sci.23;235-241
- Terada,M.,Mizuhashi, F. Murata, K. and Tomita,T. (1999).
Mepanipyrim, a new fungicide, inhibits intrcellular transport of
very low density lipoprtein in rat hepatocytes.Toxicology and
applied pharmacology 154,1-11.
- Baron,D.N.(1984) . A short textbook of chemical pathology.
ELBS,5
th
ed. The English language book society and holder and
Stoughton, pp.188-206.
- Urmila M. Joshi and Arvind K. Desal, (1988). Biochemical
changes in the liver of fish, Tilapia mossambica (peters), during
continuous exposure to Monocrotophos. Ecotoxicologyand
Environmental safety 15, 272-276.
- Pittz, E.P., Rourke D., Abraham, R. And Conlston F. (1979)
Alteration in hepatic microsomal proteins of mice administered
Hirex orally. Bull. Environ. Toxicol. 1, 21 (3): 344-351.
- Kniewald J. Perizovic M., Gomerac T. Milkovic K. And
Kniewald Z,(1987).Indirect influence of striazine on rat
gonadotropic mechanism at early post natal period. Journal of
Steroid Biochemistry 27, 1095 - 1100
- Dikshith and Raizada,(1983). Response of Carbon
Tetrachloride Pretreated Rats to Endosulfan, Carbaryl and
Phosphamidon. Industrial Health, 21; 263 – 271.