ASSESSMENT OF THE CHANGES IN SOME DIAGNOSTICS ENZYMES AND OTHER PARAMETERS IN Wistar albinoRATS TREATED WITH PESTICIDES DURING GESTATION

Auteurs-es

  • D ZAMA University of Mentouri Constantine
  • Z MERAIHI University of Mentouri Constantine
  • N BOUBEKRI University of Mentouri Constantine
  • A AMRANI University of Mentouri Constantine
  • S TEBIBEL University of Mentouri Constantine
  • N BAALI University of Mentouri Constantine

Mots-clés :

organophosphorus pesticides, hepatotoxicity, teratogenic, liver function, pregnant rates

Résumé

The present work investigates the serum enzymes activities (LDH, ALP, AST, ALT, CPK) and the level of cholesterol, glucose, triglycerides, bilirubin, creatinine, and the total protein in female rats treated with pesticides; Chlorpyrifos-ethyl and Phosalone. Pregnant rats were divided into eleven groups containing 10 individuals each, and classified intothree categories were treated orally (from the 6th to the 15th day of gestation) with different doses (1/5, 1/4, 1/3, 1/2, 2/3 LD50) of both pesticides .Animals were dissected in the 19th day of gestation, and then blood samples were collected for biochemical study.
The obtained results showed that the liver is the target organ for pesticides toxicities, as indicated by the perturbations in its functions. Thus, a significant elevation in serum AST, ALT and ALP activities has been observed, while their liver activities were highly decreased. Serum cholesterol and triglycerides
levels were also decreased significantly. However, after treatment with different doses of both pesticides, the liver wet weights were highly increased, whereas, the foetus weights were significantly decreased. Generally, the degrees of observed variations were found to be dose dependent.

Bibliographies de l'auteur-e

D ZAMA, University of Mentouri Constantine

Laboratory of Animal Physiology

Z MERAIHI, University of Mentouri Constantine

Laboratory of Applied Enzymology

N BOUBEKRI, University of Mentouri Constantine

Laboratory of Animal Physiology

A AMRANI, University of Mentouri Constantine

Laboratory of Animal Physiology

S TEBIBEL, University of Mentouri Constantine

Laboratory of Animal Physiology

N BAALI, University of Mentouri Constantine

Laboratory of Animal Physiology

Références

- Simic B and Kniewald J.(1980). Effect of pesticides on

reproductive system. Acta pharmaceutica jugoslavia 30, 59-72.

- Deskin R., Rosenstein, N. Rogers and B. Westbrook (1979)

.Parathion toxicity in prenatal rats exposed in utero . toxicology

letters 3, 11-14.

- Shih, T.M., Cowan, F. and Koviak, T. (1988). Relationship

between suman-induced lethality and depression of tissue

cholinesterase (ChE) activity Fect. Am. Soc. Exp. Biol. J. 2,1791.

-Tezak Z, B. Simic and J. Kniewald (1992). Effect of

pesticides on oestradiol-receptor complex formation in rat uterus

cytosol . Fd. Chem. Toxic. , vol 30, N°10, 879-85.

-Mattsson J.L,Wilmer J.W., Shankar M.R., Berdasco

N.M.,Crissman J.W.,Maurissen J.P. and Bond D.M. (1996).

Single-dose and 13week repeated-dose neurotoxicity screenings

studies of chlopyrifos. Food.Chem. Toxicol. 34:393-405.

- Muto M.A. Lobelle F. Jr, Bidanset J.H. Wurpel J.N. (1992).

Embryo toxicity and neuro toxicity in rats associated with prenatal

exposure to DURSBAN.Vet. Hum. Toxicol. 34: 498-501.

-Dam K., Garcia S.J., Seidler F.J., Slotkin T.A.

(1999).Neonatal chlorpyrifos exposure alters synaptic

development and neuronal activity in cholinergic and

catecholaminergic pathways. Brain Res. Dev. Brain. Res. 116: 9-20.

]- Gupta J., Datta C., Sarkar A., Sengupta D. (1992). Effect of

Malathion on antioxidant defense system in human fetus-an in

vitro study. Ind. J. Exp. Biol. 30: 352-354.

-Hunter D.L., Lassiter T.L., Padilla S. (1999).Gestational

exposure to chlorpyrifos: comparative distribution of

trichloropyridinol in the fetus and dam. Toxicol. Appl. Pharmacol.

: 16-23.

-Nieves Tormo, Juan Raman Gimeno, Miguel Angel Sogorb,

Nuria Diaz-Alejo and Engenio Vilanova (1993). Soluble and

particulate organophosphorus Neuropathy target esterase in brain

and sciatic nerve of the hen cat, rat and chick. Journal of

Neurochemistry vol 61, N°6, 2165-68.

- Donald M. Maxwell, Karen M. Brecht, Bhupendra P.

Doctor and Alan D. Wolfe (1993). Comparison of antidote

protection against Soman by pyridostigmine, HI-Gand acetyl

cholinesterase. The journal of pyharmacology and experimental

therapentics vol 264, N°3. 1085-89.

- Dikshith T.S.Tandon S.K., Datta K.K., Gupta P.K. and

Behari,J.D.(1978).Comparative response of male rats to parathesis

and lindane: histopathologicaland biochemical studies.

Environmental research 17, 1-9.

]- GomesJ,Dawodu AH, Liyod O,Anilal SV(1999). Hepatic

injury and disturb amino acid metabolism in mice following

prolonged exposure to organ phosphorus pesticides. Vet. Hum.

Toxicol. 34:498-501.

- Bagchi D, Bagchi M, hassoun EA, and Stohs SJ (1995). In

vitro and in vivo generation ofreactive oxygen species, DNA

damage and lactate dehydrogenase leakage by selected

pesticides.Toxicology, 104:129-140.

- Dam K, Seidler Fj and Slotkin TA. (1998).Developmental

neurotoxicity of chlorpyrifos: delayed targeting of DNA synthesis

after repeated administration. Brain Res.Dev.Brain Res.;108 :39-45.

- Roy TS, Andrews JE,Seider FJ and Slotkin TA.(1998).

Chlorpyrifos elicits mitotic abnormalities and apoptosis in

neuroepithelium of cultured rat embryos.Teratology , 58: 62 – 86.

- Song X, Castrucci AML and Lamy-Freund MT, (1998).

Modeling the developmental neurotoxicity of chlorpyrifos in vitro:

macromolecule synthesis in PC12 cells . Toxicol. And Appl.

Pharmacol. 151:182-191.

- Chanda SM and Pope CN.(1996). Neurochemical and

neurobehavioral effects of repeated gestational exposure to

chlorpyrifos in maternal and developing rats.

Pharmacol.Biochem.Behav. 53:771-776.

- Witney KD, Seidler FJ and Slotkin TA (1995) .

Developmental neurotoxicity of chlorpyrifos : cellular

mechanism.Toxicol. And Appl. Pharmacol. 134:53-62.

- Wootton, I.D.P (1964).Micronalysis in medical

biochemistry.London: churchill.

[ 21]- Lowry, O.H. Rosebrough, N.J., Farr A.L. and Randal, R.J.

(1951). Protein measurement with folin-phenol reagent.J.

Biochem. 193,265.

- Bligh E.G. And W.J. Dyer (1959). A rapid method of total

lipid extraction and purification . can.J. Biochem. Physiol., 37-911.

- Van Handel E. and D.B. Zilversmit, (1957). Micro method

for determination of serum triglycerides. J. Lab. Clin. Med., 50,

[ 24]- Rice, E.W. (1970)Standared Method in clinical chemistry.

R.P. Mac Donald (Ed), Academie press, New York, 6, 215.

[ 25]- Rizk, G.A., Ali M.A., Sheta I.B. and Seleim Z.M. (1990).

Effect of acyl urea derivatives on the liver of mouse Mus

musculus.J. Egypt toxicol., 6: 73-88

- Seleim, Z.M. 1988.Histological and biochemical studies of

some acyl urea derivatives on white mice.Ph.D. Thesis, Pesticide,

facility of Agric. Minia Univ. PP. 242.

[ 27]- Esia, Amal A. And Selium Z.M. (1992).Effect of

Alphamethrinon hepatorenal function on mall [albino mice Mus

musculus J. Egypt. Soc. Txical. 9: 45-49.

- Kadota, T. Okuna, Kohdo, H. and Mtyamoto J. (1976).

Mammalians toxicological study ofpermethin, 3 phenoxy benzyl

(±) astrans –2,2- dimethyl-3-(2, 2 dichlorvinyl) –cyclopropane –1-

carboxylate. Botyukgoku, 41: 143-151.

- Abston P.A. and Yarbrough J.D (1976).The in vivo effects

of Mirex on soluble hepatic enzymes in the rat. Pesti. Biochem.

Physiol. , 6 (1): 192-199.

[ 30]- Robinson, K.M. and Yarbrough J.D. (1978). Liver response

to oral administration of Mirex inrates. Pest. Biochem. Physiol.8

(1): 65-72.

-Pradeep Bhatnagar and Nirmala Jain, (1986).

Morphofunctional changes in the liver of male mice after chronic

treatment with Phosphamidon. Bull.Environ. Contam. Toxicol.

, 767 – 773.

- Young M.F., Trombetta, L.D.and carson, S., (1986).Effect

of diflubenzuron on the mouse liver. J. Appl. Toxicol. 6 (5): 343-348.

-Quest,J.A., Copley,M.,P., Hamernik,K.L., Rinde,E.,

Fisher,B., Engler,R., Burnam,W.L. and Fenner-Crisp,P.A. (1990).

Evaluation of the carcinogenic potential of pesticides. - Reg.

Toxicol.12, 117-126

-Chang, J.C.,Pavkov,K.L., Campbell,W.R.,andWyand,D.S.

(1991) 90-day oral toxicity study with methidathion in beagle

dogs . Toxicologist 11,159.

[ 35]- Kiran, R.M., Bansal M. and Bansal R.C. (1988). Effect of

carbamate on some enzymes of rat liver and kidney. pesticides,

, (3), 8-10.

[ 36]- Uppal, R.P. and Ahmed A.(1977). Blood cholinesterase and

serum transaminase in Malathion toxicity in buffalo calves Ind. J.

Anim. Sci. 47 (10): 636-639

-Abd Alla, E.A.M.; Ahmed,N.S.;Hassan M.N.A. and Fouzy,

A.S.M.(1996). Malathion administration and its effect on some

liver and kidney function.Proc.of 3

rd

CTOX6DC,19-23Nov.,1995,Cairo,Vol.III,61-68.

-Abdel-Raheem, K. El-Elaimy I., El-Mossallamy N. And

Sherief N. (1984). Biochemical responses to induced intoxication

with insecticides. Effect of acute and repeated administration of

pyrethroid.Proc. Zool-Soc. A.R. Egypt. 13: 141-156.

- Saleh,N.A. and Mahmoud M.I.(1996) . Effect of sub lethal

doses of fenvealerate on certain biochemical parameters of cotton

leaf worm and male rats.3

rd

CTOX6DC,19-23 Nov.,1995

Proc.Vol.III:15-26.

-Seleim,Z.M . (1992).Hematological and biochemical studies

on white male rats exposed to two benzoylphenyl urea

compounds. J.Egyp. Soc. Toxicol. 9:75-80.

- Seleim,Z.M .(1995). Abamectin intoxication in rats and

effect on liver functions. J.Egyp. Soc.Toxicol.14:113-118.

- Terada,M.,Mizuhashi, F. Murata, K. Inoue,H. and

Tomita,T.(1998). Mepanipyrim inducesfatty liver in rats but not

mice or dogs. J. Toxicol. Sci.23;223-234

- Terada,M.,Mizuhashi, F. Murata, K. and Tomita,T.(1998).

Effect of Mepanipyrim on lipid metabolism in rats. J. Toxicol.

Sci.23;235-241

- Terada,M.,Mizuhashi, F. Murata, K. and Tomita,T. (1999).

Mepanipyrim, a new fungicide, inhibits intrcellular transport of

very low density lipoprtein in rat hepatocytes.Toxicology and

applied pharmacology 154,1-11.

- Baron,D.N.(1984) . A short textbook of chemical pathology.

ELBS,5

th

ed. The English language book society and holder and

Stoughton, pp.188-206.

- Urmila M. Joshi and Arvind K. Desal, (1988). Biochemical

changes in the liver of fish, Tilapia mossambica (peters), during

continuous exposure to Monocrotophos. Ecotoxicologyand

Environmental safety 15, 272-276.

- Pittz, E.P., Rourke D., Abraham, R. And Conlston F. (1979)

Alteration in hepatic microsomal proteins of mice administered

Hirex orally. Bull. Environ. Toxicol. 1, 21 (3): 344-351.

- Kniewald J. Perizovic M., Gomerac T. Milkovic K. And

Kniewald Z,(1987).Indirect influence of striazine on rat

gonadotropic mechanism at early post natal period. Journal of

Steroid Biochemistry 27, 1095 - 1100

- Dikshith and Raizada,(1983). Response of Carbon

Tetrachloride Pretreated Rats to Endosulfan, Carbaryl and

Phosphamidon. Industrial Health, 21; 263 – 271.

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Publié-e

2005-06-01

Comment citer

ZAMA, D., MERAIHI, Z., BOUBEKRI, N., AMRANI, A., TEBIBEL, S., & BAALI, N. (2005). ASSESSMENT OF THE CHANGES IN SOME DIAGNOSTICS ENZYMES AND OTHER PARAMETERS IN Wistar albinoRATS TREATED WITH PESTICIDES DURING GESTATION. Sciences & Technologie. C, Biotechnologies, (23), 51–56. Consulté à l’adresse https://revue.umc.edu.dz/c/article/view/563

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