QUANTITATIVE ESTIMATIONS OF POLYPHENOLIC COMPOUNDS IN VICIA FABA L. (LEGUMINOSAE) SEEDS
Keywords:
Vicia faba L, genotype, polyphenols, condensed tannins, Antinutritionnal factorsAbstract
Polyphenolic compounds and tannins are natural substances of secondary plant metabolism. They are well known for their antinutritional effects. These properties are due to their ability to interact and precipitate protein of the diet. They are responsible for the inhibition of digestion and the decrease of nutritive value of some legumes and cereals grains. Owing to their ability to interact strongly with protein of the ratio, they could be responsible to the inhibition activity of several enzymes [1,2]. So an important particularity must be reserved to these compounds when they are integrated into the diet. Five methods for the measurement of total phenolics, condensed tannins, and tanning capacity of Vicia faba L. were tested for their reliability ; some of them were improved for their sensitivity. The results were interpreted so as to draw the phenolic pattern of each variety of Vicia faba L, and they revealed different cases.References
- Griffits D.W., "The inhibition of digestive enzyme by polyphenolic compounds. Nutritional and toxicological significance of enzyme inhibitors in foods", (1986) pp. 509-515. Ed. by Mendel Fiedman.
- Butler L.G., "Protein-polyphenol interaction: nutritional aspects", Proceeding of the XVIth international conference of group polyphenol, Portugal, Vol. 16, (1992), pp. 11-18.
- Price M.L., Butler L.G., "Rapid visual estimation and spectrophotometric determination of tannin content sorghum grain", J. Agri. Food chem., Vol. 25, (1977), pp. 1267-1276.
- Bate-smith E.C., "Phytochemistry of proanthocyanidins. Phytochemistry", Vol. 14, (1975), pp. 1107-1113.
- Marigo G., "Sur une méthode de fractionnement et d’estimation des composés phénoliques chez les végétaux", Analusis, Vol. 2, (1976), pp. 106-110.
- Price M.L., Butler L.G., "A critical evaluation on the vanillin reaction as an assay for tannins Sorghum grain", J. Agric Food Chem.,Vol. 2, (1978), pp. 1214-1218.
- Bate-Smith E.C., "Astringent tannins of the leaves of Geranium species", Phytochemistry, Vol. 20, (1981), pp. 211-216.
- Broadhurst R.B., Jones W.T., "Analysis of condensed tannins using acidified vanillin", J.Sci. Food Agric., Vol. 29, (1978), pp. 784-788.
- Mole S., Waterman P.G., "A critical analysis of techniques for measuring tannins in ecological studies", Oecologica, Vol. 72, (1978), pp. 137-147.
- Porter L.G., Woordruffe J., "Heamanalysis: the relative astringency of proanthocyanidins polymers", Phytochemistry, Vol. 23, (1984), pp. 1255-1256.
- Prieur C. Rigaud J., Moutounet M., "Oligomeric and polymeric procyanidins from grape seeds", Phytochemistry, Vol. 36, (1994), pp. 781-784.