IMMUNOHISTOCHEMICAL EVIDENCE FOR XANTHINE OXIDOREDUCTASE IN HUMAN MYOCARDIUM

Authors

  • M BENBOUBETRA University of Sétif
  • M TAVENIER Erasmus University Rotterdam (EUR) The Netherlands
  • T H VAN DER KWAST Clinical Pathology, EUR
  • N AL-MUHTASEB University of Petra, Amman
  • R HARRISON University of Bathm UK
  • HARI S SHARMA Pharmacology, EUR, Netherlands
  • J W DE JONG Erasmus University Rotterdam (EUR) The Netherlands

Keywords:

Explanted heart, Immunohistochemistry, Monoclonal Antibodies, Reactive Oxygen Species (ROS), and Xanthine oxidoreductase (XOR)

Abstract

Xanthine oxidoreductase (XOR) could be an important source of reactive oxygen species (ROS) in the heart. The enzyme is clearly active in rat heart but its present in the human heart is controversial. This study was to detect XOR immunohistochemically in human myocardium using mouse raised monoclonal antibodies to purified human and bovine milk XOR. We assayed the presence of the enzyme in explanted human heart and compared to rat heart results. Using positive and negative controls, immunoactive XOR in heart was clearly present in the cardiomyocytes and in the smooth muscle cells of blood vessels in both species. The endothelial cells showed no staining in the smaller blood vessels. Other data suggest that ROS generating desulpho-type of XOR,  present in rat heart,  inactive with the substrate (hypo)xanthine is active using  NADH as substrate, producing ROS. We speculate that this type of XOR plays a role in radical-induced cardiovascular disease.

Downloads

Download data is not yet available.

Author Biographies

M BENBOUBETRA, University of Sétif

Laboratory of Applied Biochemistry
Faculty of Sciences

M TAVENIER, Erasmus University Rotterdam (EUR) The Netherlands

Thorax Centre

N AL-MUHTASEB, University of Petra, Amman

Faculty of Pharmacy and Medical Technology

R HARRISON, University of Bathm UK

Biochemistry

J W DE JONG, Erasmus University Rotterdam (EUR) The Netherlands

Thorax Centre

References

- Wiezorek J.S., Brown D.H., Kupperman D.E. and Brass C.A., J. Clin. Invest., 94, (1994), pp. 2224-2230

- Nishino T., J. Biochem., 116, (1994), pp. 1-6.

- Huizer T., Dejong J.W., Nelson J.A., Czarnecki W., Serruys P.W., Bonnier J.R.M. and Troquay R., J. Mol. Cell Cardiol., 21, (1989), pp. 619-695.

- Vlessis A.A., Ott G. and Cobanoglu A. J. Thorac. Cardiovasc. Surg., 107, (1994), pp. 482-486.

- Zweier J.L, Kuppusamy P., Thompson-Groman S., Klunk D. and Lutty G.A., Am. J. Physiol., 266, (1994), pp. C700-C708.

- DeJong J.W., Huizer T., Janssen M., Krams R., Tavenier M. and Verdouw P.D., In: Piper H.M., Preusse C.J., eds. Ischemia-Reperfusion in cardiac surgery. Dordrecht: Kluwer Acad. Publ., (1993), pp. 295-315.

- DeJong J.W., Van der Meer P., Nieukoop A.S., Huizer T., Stroeve R.J. and Bos E., Cir. Res., 67, (1990), pp. 770-773.

- Muxfeldt M. and Schaper W., Basic Res. Cardiol., 82, (1987), pp. 486-492.

- Janssen M., Van der Meer P. and De Jong J.W., Cardiovasc. Res., 27, (1993), pp. 2052-2057.

- Eddy L.J., Stewart J.R., Jones H.P., Engerson T.D., McCord J.M. and Downey J.K., Am. J. Physiol., 253, (1987), pp. H709-H711.

- Grum C.M., Gallagher K.P., Kirsh M.M. and Shlafer M., J. Mol. Cell Cardiol., 20, (1989), pp. 263-267.

- Podzuweit T., Beck H., Muller A., Bader R., Gorlach G. and Scheld H.H., Cardiovasc. Res., 25, (1991), pp. 820-830.

- Wajner M. and Harkness R.A., Biochim. Biophys. Acta., 991, (1989), pp. 79-84.

- Hellsten-Westing Y. Histochemistry, 100, (1993), pp. 215-22.

- Jarasch E-D., Bruder G. and Heid H.W. Acta Physiol. Scand., Suppl., 548, (1986), pp. 39-46.

- MacGowan S.W., Regan M.C., Malone C., Sharkey O., Young L., Gorey T.F. and Wood A.E. Ann. Thorac. Surg., 60, (1995), pp. 1289-1293.

- Moriwaki Y., Yamamoto T., Suda M., Nazako Y., Takahashi S., Agbedena O.E., Hada T. and Higashino K., Biochim. Biopys. Acta., 1167, (1993), pp. 327-330.

- Kooij A., Schijins M., Frederiks W.M., Van Noorden C.J.F. and James J., Virchows Arch [B] Cell Pathol., 63, (1992), pp. 17-23.

- Swain J. and Gutteridge J.M., FEBS Lett., 368, (1995), pp. 513-515.

- Griendling K.K. and Ushio-Fukai M., Trends Cardiovasc. Med., 7, (1997), pp. 301-307.

- Suzuki Y.J. and Ford G.D., J. Mol. Cell. Cardiol., 31, (1999), pp. 345-353.

- Griendling K.K., Sorescu D. and Ushio-Fukai M., Circ. Res., 86, (2000), pp. 494-501.

- Millar T., Stevens C.R., Benjamin N., Eisenthal R., Harrison R. and Blake D.R., FEBS Lett., 427, (1998), pp. 225-228.

- Godber B.L.J., Doel J.J., Sapkota G.P., Blake D.R., Stevens C.R., Eisenthal R. and Harrison R., J. Biol. Chem., 275, (2000), pp. 7757-7763.

- Moncada S.M., Palmer R.M.J. and Higgs E.A., Pharmacol. Rev., 43, (1991), pp. 109-142.

- Farrell A.J. and Blake D.R., Ann. Rheum. Dis., 55, (1996), pp. 7-20.

- Stamler J.S., Cell, 78, (1994), pp. 931-936.

- Rouquette M., Page S., Bryant R., Benboubetra M., Stevens C.R., Blake D.R., Which D., Harrison R. and Tosh D., FEBS Lett., 426, (1998), pp. 397-401.

- Abadeh S., Killacky J., Benboubetra M. and Harrison R., Biochim. Biophys. Acta., 1117, (1992), pp. 25-32.

- Benboubetra M., Gleeson A., Harris C.P.D., Khan J., Arrar L., Brennand D., Reid J.D., Reckless J.D., Harrison R., Eur. J. Clin. Invest.; 27, (1997), pp. 611-619.

- Page S., Powell P., Benboubetra M., Stevens C.R., Blake D.R., Selase F., Wolstenholme A. and Harrison R., Biochim. Biophys. Acta, 1381, (1998), pp. 191-202.

- Guesdon J-L., Ternynck T. and Avrameas S., J. Histochem. Cytochem., 27, (1979), pp. 131-1139.

- Sarnesto A., Linder N.and Raivio K.O., Lab. Invest., 74, (1996), pp. 48-56.

- Terada L.S., J. Mol. Cell. Cardiol., 26, (1994), pp. 25-132.

- Massey V. and Edmondson D., J. Biol. Chem., 245, (1970), pp. 6595-6598.

- Ikegami T. and Nishino T., Arch. Biochem. Biophys., 247, (1986), pp. 254-260.

- Bray R.C., in ‘Boyer PD, ed. The Enzymes, 3rd ed., vol. 12, New York, Academic Press, (1975), pp. 299-419.

- Aminlari M., Gilanpour H., Taghavianpour H. and Veseghi T., Comp. Biochem. Physiol. C., 92, (1989), pp. 259-262.

- Buzaleh A.M., Vazquez E.S. and Battle A.M., Gen. Pharmacol., 20, (1989), pp. 323-327.

- Nishino T., Usami C. and Tsushima K., Proc. Natl. Acad. Sci. USA., 80, (1983), pp. 1826-1829.

- Sanders S.A., Eisenthal R. and Harrison R., Eur. J. Biochem., 245, (1997), pp. 541-548.

- Iademarco M.F., Barks J.L. and Dean D.C., J. Clin. Invest., 95, (1995), pp. 264-271.

- Fukushima T., Adachi T. and Hirano K., Biol. Pharm. Bull., 18, (1995), pp. 156-158.

- Adachi T., Fukushima T., Usami Y. and Hirano K., Biochem. J., 289, (1993), pp. 523-527.

Downloads

Published

2002-12-01

How to Cite

BENBOUBETRA, M., TAVENIER, M., VAN DER KWAST, T. H., AL-MUHTASEB, N., HARRISON, R., SHARMA, H. S., & DE JONG, J. W. (2002). IMMUNOHISTOCHEMICAL EVIDENCE FOR XANTHINE OXIDOREDUCTASE IN HUMAN MYOCARDIUM. Sciences & Technology. A, Exactes Sciences, (18), 91–95. Retrieved from https://revue.umc.edu.dz/a/article/view/1817

Issue

N° 18 Décembre 2002

Section

Articles

Similar Articles

You may also start an advanced similarity search for this article.