IMMUNOHISTOCHEMICAL EVIDENCE FOR XANTHINE OXIDOREDUCTASE IN HUMAN MYOCARDIUM

Auteurs-es

  • M BENBOUBETRA University of Sétif
  • M TAVENIER Erasmus University Rotterdam (EUR) The Netherlands
  • T H VAN DER KWAST Clinical Pathology, EUR
  • N AL-MUHTASEB University of Petra, Amman
  • R HARRISON University of Bathm UK
  • HARI S SHARMA Pharmacology, EUR, Netherlands
  • J W DE JONG Erasmus University Rotterdam (EUR) The Netherlands

Mots-clés :

Explanted heart, Immunohistochemistry, Monoclonal Antibodies, Reactive Oxygen Species (ROS), and Xanthine oxidoreductase (XOR)

Résumé

Xanthine oxidoreductase (XOR) could be an important source of reactive oxygen species (ROS) in the heart. The enzyme is clearly active in rat heart but its present in the human heart is controversial. This study was to detect XOR immunohistochemically in human myocardium using mouse raised monoclonal antibodies to purified human and bovine milk XOR. We assayed the presence of the enzyme in explanted human heart and compared to rat heart results. Using positive and negative controls, immunoactive XOR in heart was clearly present in the cardiomyocytes and in the smooth muscle cells of blood vessels in both species. The endothelial cells showed no staining in the smaller blood vessels. Other data suggest that ROS generating desulpho-type of XOR,  present in rat heart,  inactive with the substrate (hypo)xanthine is active using  NADH as substrate, producing ROS. We speculate that this type of XOR plays a role in radical-induced cardiovascular disease.

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Bibliographies de l'auteur-e

M BENBOUBETRA, University of Sétif

Laboratory of Applied Biochemistry
Faculty of Sciences

M TAVENIER, Erasmus University Rotterdam (EUR) The Netherlands

Thorax Centre

N AL-MUHTASEB, University of Petra, Amman

Faculty of Pharmacy and Medical Technology

R HARRISON, University of Bathm UK

Biochemistry

J W DE JONG, Erasmus University Rotterdam (EUR) The Netherlands

Thorax Centre

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Publié-e

2002-12-01

Comment citer

BENBOUBETRA, M., TAVENIER, M., VAN DER KWAST, T. H., AL-MUHTASEB, N., HARRISON, R., SHARMA, H. S., & DE JONG, J. W. (2002). IMMUNOHISTOCHEMICAL EVIDENCE FOR XANTHINE OXIDOREDUCTASE IN HUMAN MYOCARDIUM. Sciences & Technologie. A, Sciences Exactes, (18), 91–95. Consulté à l’adresse https://revue.umc.edu.dz/a/article/view/1817

Numéro

N° 18 Décembre 2002

Rubrique

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