Effect of N fertilization on storage protein and some amino acid quantities of two Tunisian Barley varieties differing in their degrees of adaptation to environmental conditions
الملخص
Two Tunisian barley varieties,Martin and Rihane, differing in degrees of adaptation to environmental conditions, were grown under different treatments ofnitrogen (N) fertilization. Total nitrogen content increased when intensifying N supply whereas grain and albumen weights decreased. Effect of N fertilization on storage protein components (expressed in mg per albumen) was determined by Nu-PAGE electrophoresis system using the MOBS running buffer and densitometry analysis. The effect of N supply on the change of B-hordein fraction and some amino acids (AA) particularly the cysteine, implicates in disulfide bonds of protein aggregates,
was discussed in relation to different degrees of adaptability toenvironmental conditions which characterize each barley variety.
المراجع
-Autran, J.C., Halford, N., Shewry, P.R.,. 2001. Nitrogen and seed storage proteins: The biochemistry and molecular biology of seed storage proteins. In: The nitrogen assimilation by plants (J.F. Morot-Gaudry and P. Lea, eds.) Springer. Verlag, 295-341.
-Ayoub, M., Guertin, S., Lussier, S., 1994. Timing and level of nitrogen fertility. Effects on spring wheat yield in eastern Canada. Crop. Sci. 34: 748-756.
-Bettaieb-Ben Kaâb, L., Attias, J., 1992. Electrophoresis
heterogeneity of the hordein five barley (Hordeum vulgareL.) cultivars from Tunisia. C.R. Acad. Sci. Paris, Serie III. 314: 141-146.
-Bindlingmeyer, B.A., Cohen, S.A., Tarvin, T.L., 1984.
Rapid analysis of amino acids using pre-column derivatization. J.Chromatogr. 336: 93-104.
-Brandt, A., Montembault,A., Cameron-Mill, V., Rasmussen, S.K., 1985. Primary structure of a B1 hordein gene from barley. Carlsberg. Res. Commun. 50: 333-345.
-Brukner, P.L., Morey D.D., 1988. Nitrogen effects on soft red winter wheat yield, agronomic characteristics and quality. Crop. Sci. 23: 152-157.
-Daâloul A., 1996. Les resources phytogénétiques des
cereales en Tunisie. Séminaire sur la conservation et la valorisation des ressources génétiques des plantes du Maghreb. Projet FEM/PNUD/FAO, 12-14 Mars 1996. Sidi Thabet, Tunisie.
-Daniel, C., Triboi, E., 2000. Effects of temperature and
nitrogen nutrition on the grain composition of winter wheat: effects on gliadin content and composition . J. Cereal. Sci. 32: 45-56.
-Eynard L., Laurière M., 1988. The combination of Indian ink staining with immuno- chemiluminescence detection allows precise identification of antigens on blots. Application to the study of glycosylated barley storage proteins. Electrophoresis.19: 1394-1396.
-Fatta N., Caputo, C., Barneix, A.J., 2000. The absence
of the short arm of chromosome 7B Produces inhibition of N mobilization and decreases grain protein concentration in wheat (Triticum aestivumL.) cv. Chinese Sp. Agronomie. 20: 423-430.
-Giese, H., Andersen, B., Doll, H., 1983. Synthesis of major storage protein, hordein, in barley. Planta. 159: 60-65.
-Gravelle, W.D., Alley, M.M., Brann, D.E., Joseph, K.D.S.M., 1988. Split nitrogen application effects on yield, lodging and nutrient uptake of soft red winter wheat. J. Prod. Agric.1: 249- 256.
-Jia, Y.Q., Fabre, J.L., Aussenac, T., 1996a. Effects of
growing location on response of protein polymerization to increase nitrogen fertilization for common wheat cultivars season: Relationship with some aspects of the bread making quality. Cereal. Chem. 73: 526-532.
-Jia, Y.Q., Masbou, V., Aussenac T., Fabre J., Debaeke, P., 1996b. Effects of nitrogen fertilization and maturation conditions on protein aggregates and the bread making quality of season, a common wheat cultivars. Cereal. Chem. 73: 123-130.
-Laemmli, U.K., 1970. Cleavage of structural proteins
during the assembly of the heads of bacteriophage T4. Nature (London). 227: 680-685.
-Martin, B.J., Sutton, K.H., Moyle T.H., Hay, R.L., Gillespie, R.N., 1992. Effect of nitrogen Fertilizer on the yield and quality of six cultivars of autumn-sown wheat. N.Z.J. Crop. Hortic. Sci. 20: 273-282.
-Mullen, R.W., Freeman, K.W., Raun, W.R., Johnson, G.V., Stone, M.L., Solie, J.B., 2003. Identifying an Inseason response index and the potential to increase wheat yield with nitrogen. Agronomy J. 95: 347-351.
-Roth, G.W., Marshall, H.G., 1987. Effects of timing of nitrogen fertilization and a fungicide on softres winter wheat. Agron J. 79: 197-200.
-Scheromm, P., Martin, G., Bergoin, A., Autran, J.C., 1992. Influence of nitrogen fertilization on the
potential bread-baking quality of two wheat cultivars differing in their response to increasing nitrogen supplies. Cereal. Chem. 69: 664-670.
-Shewry, P.R., Miflin, B.J., 1982. Gene for storage proteins of barley. Plant Foods For Human Nutrition. 31: 251-267.
-Shewry, P.R., Miles, M. J., Tatham, A.S., 1994. The prolamin storage proteins ofwheat and related cereals. Prog. Biophys. Molec. Biol. 61: 37-59.
-Wieser, H., Seilmeier, W., 1998. The influence en
fertilization on quantities and proportions of different protein types in wheat flour. J. Sci. Food. Agric. 76: 49-55.
-Zebarth, B.J., Sheard, R.W., 1992. The influence of rate and timing of nitrogen fertilization on yield and quality of hard red winter wheat in Ontario. Can. J. Plant. Sci. 72:13-19.
