CHARACTERIZATION OF STREPTOMYCES SCABIES ISOLATED FROM COMMON SCAB LESIONS ON POTATO TUBERS BY MORPHOLOGICAL, BIOCHEMICAL AND PATHOGENICITY TESTS IN CHLEF REGION IN WESTERN ALGERIA
Keywords:
Streptomyces scabies, Common scab, Potato, biochemical characteristics, AlgeriaAbstract
In summer 2002, nine isolates causing common scab symptoms were collected from tubers presenting superficial and raised corky lesions on tubers surface in number of locations from the Chlef region in Algeria.The common scab inducing organismwere characterized by creamy colonies on yeast malt extract (YMA) and by arial mycelium which turned brown with age.
The organism was Gram positive, non motile, utilized L-arabinose, D-fructose, D-glucose and rhamnose. They degraded the xylose and starch with production of melanin on peptone yeast extract agar-iron(PYI).
Additionally, it was determined that most strains obtained from differents locations were identical in morphology and biochemical characteristics. Furthermore, these isolatesshares most pattern with the
reference isolate ATCC49173.
Results of pathogenicity tests showed that all isolates were pathogenic on both cultivars(Desiree and Claustar) causing symptoms with aggressiveness of strains varying from mild to moderately severe. Koch’s postulates for isolates were also fulfilled.
References
- Afek, u., Orenstein, J., 2002- Desinfecting potato tubers using steam treatments. Canadian Journal of Plant Pathology 24: 36-39.
-Bang, H., 1995- Effects of soil conditions on the prevalence of netted scab. Acta Agriculturae Scandinavica 45: 271-277.
-Bouchek- Mechiche, K., Pasco, C., Andrivon, D., Jouan, B., 2000- Differences in host range, pathogenicity to potato cultivars and response to soil temperature among Streptomyces species causing common and netted scab in France.
-Elesawy, A.A., Szabo, I.M., 1979- Isolation and characterization of Streptomyces scabies strains from scab lesions of potato tubers. Designation an neotype strain of Streptomyces scabies. Acta Academiae Scientifica Hungarica 26: 311-320.
-Faucher, E., ¨rados, E., Goyer, C., Hodge, N., Hogue, R., Stall, R.E., Beaulieu, C., 1995-Characterization of Streptomycetes causing deep pitted scab of potato in Quebec, Canada. International Journal of Systematic Bacteriology 46: 635-639.
-Goth, R.W, Haynes, K.G, Wilson, D.R., 1993- Evaluation and characterization of advanced potato clones for resistance to scab by cluster analysis.Plant Disease 77:911-914.
-Goth, R.W., 1965- Puncture method for isolating bacterial blights of bean. Phytopathology. 55:930-931.
-Goyer, C., Beaulieu, F., 1997- Host range of Streptomycetes strains causing common scab.PlantDisease. 81:901-904.
-Goyer, c., Otrysko, B., Beaulieu, C., 1996- Taxonomic studies on Streptomycetes causing potato common scab : a review. Canadian Journal of Plant Pathology 18: 107-113.
-Gregerson, T., 1978- Rapid method for distinction of Gram negative from Gram positive bacteria. Eur.J.Appl. Microbiol.Biotechnol.5 :123-127.
-Hooker, W.J., 1981- Common scab. Pages 33-34 in : Compendium of potato diseases. American Phytopathological Society, St.Paul, MN.125pp.
-Horsfall, J.G., Barratt, R.W., 1945- An improved grading system for measuring plant disease(Abstr) Phytopathology.35:655.
-James, G., 1971- A manual of assessment keys for plant diseases. Can.Dep.Agric.Publ.1458:32-33.
-King, R.R., Lawrence, C.H., Caalhoun, L.A.1991- Correlation of phytotoxin production with pathogenicity of Streptomyces scabies isolates from scab infected potato tubers. American Potato Journal 68: 675-680.
-Kreuze, J.F., Suomalainen, S., Paulin, L., and Valkonen, J.P.T, 1999- Phylogenitic analysis of 16SrRNA genes and PCR analysis of the common scab, and netted scab in Finland.
-Lambert, D.H., and LORIA, R., 1989a. Streptomyces scabies. Sp.nov.nom. International Journal of Systematic Bacteriology 39: 387-392.
-Lambert, D.H., and LORIA, R., 1989b. Streptomyces acidoscabies. Sp.nov.nom. International Journal of Systematic Bacteriology 39: 393-396.
-Lehtonen, M.J., Rantala, H., Kreuze, J.F., Bang, H., Kuisma, L., Koski, P., Virtonen, E., Vihlman, K., Valkonen, J.P.T., 2004- Occurrence and survival of potato scab pathogens (Streptomyces species) on tuber lesions : quick diagnosis based on a pcr- based assay. Plant pathology 53 : 280-287.
-Lindholm ,P., Kortemaa, H., Kokkala, M., Haahtela, M.K.,Salkinoja- Salonen, M., Valkonen, J.P.T., 1997- Streptomyces spp isolated from potato scab lesions under nordic conditions in Finland. Plant Disease 81: 1317-1322.
-Mac faddin, J.F., 1976- Biochemical tests for identification of medical bacteria. Williams&Wilkins.Co., Baltimore, MD.307p.
-Miyajima, K., Tanaka, F., Takeuchi, T., Kuninaga, S.1998- Streptomyces turgidiscabies sp.nov. International Journal of Systematic Bacteriology 48: 495-502.
-Park, D.H., Kim J.S., Kwon, S.W., Wilson, C., Yu, Y.M., Hur, J.H., Lim, C.K., 2003- Streptomyces Luridiscabiei sp.nov., Streptomyces punisciscabiei sp.nov. and Streptomyces niveiscabiei sp.nov., which cause potato common scab disease in Korea. International Journal of Systematic and Evolutionary
Microbiology 53: 2049-2054.
-Schaad, N.N., Jones, J.B., Chun, W., eds. Laboratory guide for the identification of plant pathogenic bacteria.American Phytopathological Society, St.Paul, MN.
-Scholte, K., 1989- The effect of netted scab (streptomyces spp.) and Verticillium dahliae on growth and yield of potato. Potato Research 32: 65-73.
-Scholte, K., labruyère, R.E., 1985- Netted scab, A new name fo an old disease in Europe. Potato Research 28: 443-448.
-Shirling, E.B., Gottlieb, D., 1966- Methods for characterization of Streptomyces species. International Journal of Systematic Bacteriology 16: 313-340.
-Takeuchi, T., Sawada, H., Tonaka, F., Madsuda, I.1996- Phylogenetic analysis of Streptomyces spp. causing potato scab based on 16 S.rRNA sequences. International Journal of Systematic Bacteriology 46: 476-479.
-Wanner, L.A.,2004-Field isolates of Streptomyces differ in pathogenicity and virulence on radish. Plant disease 88:785- 796.
-Wanner, L.A., 2007-A new strain of Streptomyces causing common scab in potato. Plant Disease, 91: 352-359.
