THE EFFECT OF THERMIC PRETREATMENT AND ANTIBIOTICS ON THE SELECTIVE ISOLATION OF THE CULTURABLE ACTINOMYCETES FROM THE ALGERIAN DESERT SOIL

Auteurs-es

  • A BOUDEMAGH Université Constantine 1
  • K BENSOUICI Université Abbes Laghrour, Khenchela

Mots-clés :

Actinomycetes, Desert soil, Thermic pretreatment, Selectif medium

Résumé

In order to define the isolation conditions of actinomycetes from desert soils, the soils of the town of Biskra, situated at the gate of the Algerian Sahara was analyzed. Two types of samples have been prepared; the firt one was heated up at 110 °C during 10 minutes and the second did not undergo any heat processing. The isolation was carried out from these two types of soils on the starch casein medium added to a combination of several antifungal and antibacterial agents. Five different isolation mediums have been tested in this study. The results showed that the pretreatment of the soil with heat is not recommended. Indeed, the number of isolated actinomycetes was 38. 104 cfu.g-1 when the soil samples were heated up and 92.104 cfu.g-1 when no heating was used. The thermic processing reduces then the actinomycetes by more than 50 %. The antifungal and antibacterian which permit the elimination of undesirable germs are the nystatin and the nalidixic acid with respective concentrations of 50 µg/ml and 10 µg/ml. According to these results, the most favorable isolation medium for actinomycetes of desert soils studied is Glucose-yeast extract-malt (GLM).

Bibliographies de l'auteur-e

A BOUDEMAGH, Université Constantine 1

Laboratoire de biologie et santé, Département des sciences de la nature et de la vie.

K BENSOUICI, Université Abbes Laghrour, Khenchela

Institut des sciences de la nature et de la vie

Références

Agate A.D., Bhat J.V., (1963).A method for the preferential isolation of actinomycetes from soils. Antonie Van leeuvenhoek. 29: 297-304.

Ara I., Burhari Nadjat A, Wijayanti D.R. and Bakir M.A. (2012). Proteolytic activity of alkaliphilic salt-tolerant actinomycetes from various regions in Saudi Arabia. African journal of biotechnology 11(16):3849-3857.

Athalye M., Lacey J., and Goodfellow M., (1981). Selective isolation and enumeration of actinomycetes using rifampicin. J. Appl. Bacteriol. 51: 289-297.

Badji B., Riba A., Mathieu F., Lebrihi A., Sabaou N., (2005). Activité antifongique d’une souche d’Actinomadura d’origine Saharienne sur divers champignons pathogènes et toxinogènes. J.Myco.Méd., 15(4): 211-219.

Barakate M., Ouhdouch Y., Oufdou K.H., and Beaulieu C., (2002) Charachterization of rhizospheric soil streptomyces from Maroccan habitats, and their antimicrobial activities. Word J. Microbiol. Biotechnol. 18 : 49-54.

Boudemagh A., Kitouni M., Boughachiche H., Hamdiken L., Oulmi L., Rheghioua S. Zerizer H., Couble A., Mounier D., Boulahrouf A. and Boiron P. (2005). Isolation and mlecular identification of actinomycetes microflora of some saharian soils of South-East Algeria (Biskra, El-Oued and Ouargla). Study of antifungal activity of isolated strains. J. Med. Mycol. 15: 39-44.

Cavala M., and Eberlin T., (1994). Isolement des streptomycètes du sol. L’opéron. 4: 13-7.

Dulaney E.L., Larsen H.A., Stapley E.O., (1955). A note on the isolation of microorganisms from natural sources. Mycologia. 47: 420.

El-Nakeeb M.A., Lechevalier H.A., (1963). Selective isolation of aerobic actinomycetes.Appl Microbiol. 11:75-7.

Fan LX, Zheng JP, Yang XH (2010). The effect of natural air-dry time on actinomycetes isolation from sample soil. J Hainan Med Coll 16(3):280-284.

Hacene, H., Sabaou, N., Bounaga, N., Lefevre, G., (1994). Screening for non-polyenic antifungal antibiotics produced by rare Actinomycetales. Microbios. 79: 81-5.

Hozzein W., Ali M., Rabie W., (2008). A new preferential medium for enumeration and isolation of desert actinomycetes. World J Microbiol Biotechnol 24:1547-552.

Jung Yeop Lee, Byung Kook Hwang, (2002). Diversity of antifungal actinomycetes in various vegetative soils of Korea. Can. J. Microbiol. 48: 407- 417.

Kuster, E., Williams, S.T., (1964). Selection of media for isolation of streptomycetes. Nature. 202:928-929.

Kyng Man You, Yong Keun Park. (1996). A new method for selective isolation of actinomycetes from soil. Biotechnol. Tech. 10: 541-546.

Larpent J.P., Sanglier J.J., (1989). Biotechnologie des antibiotiques. Masson (Ed.), Paris., 481 p.

Nomura H., Ohara Y., (1969). The distribution of actinomycetes, in soil. VI. A selective plate-culture isolation method for Microbispora and Streptosporangium strains.Part 1. J. Ferment. Technol. 47: 463-469.

Nonomura H., Hayakawa. (1988). New methods for selective isolation of soil Actinomycetes. In “ Okami y., Beppu T. and ozawara H., (Ed.) Biology of actinomycetes. Japan scientific societies Press Tokyo, 288-293.

Okami, Y., and Hotta, K., (1988). Search and discovery of new antibiotics. In: Actinomycetes in biotechnology. Edited by Goodfellow M, Williams ST and Mordarski M. Academic press Ltd. London, 33-67.

Okoro C.K., Brown R., Jones A.L, Andrews B.A., Asenjo j.A., Goodfellow M., Bull A.T. (2009). Diversty of culturable actinomycetes in hyper arid soils of the Atacama Desert, Chile. Antonie-van Leeuwanhoek 95 (2):121-33.

Ouhdouch, Y., Barakate, M., and Finance, C., (2001). Actinomycetes of marocan habitats: isolation and screening for antifungal activities. Eur. J. Soil. Biol. 37: 69-74.

Palleroni, N.J. (1980). A chemotactic method for isolation of Actinoplanaceae. Arch. Microbiol. 128 : 53-55.

Pochon, J., and Tardieux, P., (1962). Techniques d’analyse en microbiologie du sol. Tourtourelle, Saint-Mandé, (Ed.).

Porter, J.N., Wilhelm, J.J., and Tresner, H.D., (1960). Method for the preferential isolation of actinomycetes from soil. Appl. Microbiol. 8: 174.

Pisano, M.A., Sommer, M.J., and Lopez M.M. (1986). Application of pretreatments for the isolation of bioactive actinomycetes from marine sediments. App Microbiol Biotechnol 25: 285-288.

Shearer, M.C., (1987). Methods for the isolation of non-streptomycete actinomycetes. J. Ind. Microbiol. 28: 91-97.

Takahashi y. Omura S. (2003). Isolation of new actinomycetes strains for the screening of new bioactive compounds. J.Gen.Appl.Microbiol. 49 (3): 141-54.

Takizawa, M., Colwell, R.R., Hill R., T., (1993). Isolation and diversity of actinomycetes in the chesapeake bay. Appl. Environ. Microbiol. 59: 997-1002.

Williams, S.T., Davies, F.L., (1965). Use of antibiotics for selective isolation and enumeration of actinomycetes in soil. J. Gen. Microbiol. 38:251-261.

Williams, S.T., Wellington, E.M.H., (1982). Principales and problems of selective isolation of microbes. In: Bioactive microbial products: Search and discovery. Academic Press, London, 9-26.

Zakharova, O. S., Renova, G.M., Zvyagintsev, D.G., (2003). Some approaches to the selectives isolation of actinomycetes of the genus actinomadura from soil. Microbiology. 72: 110-113.

Zitouni, A., Lamari, L., Boudjelale, H., Badji, B., Sabaou, N., Gaouar, A., Mathieu, F., Lebrihi, A., and Labeda, D.P., (2005). Saccharothrix algeriensis sp. Nov., isolated from a Sahara soil. Int. J. Syst. Evol. Microbiol. 54 : 1377-1381.

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Publié-e

2014-06-01

Comment citer

BOUDEMAGH, A., & BENSOUICI, K. (2014). THE EFFECT OF THERMIC PRETREATMENT AND ANTIBIOTICS ON THE SELECTIVE ISOLATION OF THE CULTURABLE ACTINOMYCETES FROM THE ALGERIAN DESERT SOIL. Sciences & Technologie. C, Biotechnologies, (39), 25–32. Consulté à l’adresse https://revue.umc.edu.dz/c/article/view/1538

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